Document Detail

Protein kinase C delta and eta differently regulate the expression of loricrin and Jun family proteins in human keratinocytes.
MedLine Citation:
PMID:  20184865     Owner:  NLM     Status:  MEDLINE    
Barrier function of the epidermis is maintained by precise expression of keratinocyte-specific structural proteins to form the cornified cell envelope (CE). Loricrin, a major component of the CE, is expressed at the late stage of keratinocyte differentiation. In this study, we reveal the isoform-specific function of protein kinase C (PKC) in the regulation of loricrin expression. Both PKCdelta and PKCeta have been recognized as differentiation-promoting isoforms. However, loricrin expression was inversely controlled by PKCdelta and PKCeta in cultured keratinocytes and 3D skin culture; i.e. loricrin expression was decreased by PKCdelta and increased by PKCeta. To clarify the mechanisms that PKCdelta and PKCeta oppositely regulate the loricrin expression, we examined the expression of activator protein-1 (AP-1) family proteins, which modulate the transcription of loricrin and are downstream molecules of PKC. PKCdelta decreased c-Jun expression, whereas PKCeta increased JunD, which are positive regulators of loricrin transcription. These findings suggest that inverse effects of PKCdelta and PKCeta on loricrin expression attributes to the expression of c-Jun and JunD.
Nagisa Kamioka; Tomoko Akahane; Yoko Kohno; Toshio Kuroki; Masafumi Iijima; Ikuo Honma; Motoi Ohba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-23
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  394     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  106-11     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Institute of Molecular Oncology, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
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MeSH Terms
Cells, Cultured
Gene Expression Regulation*
Keratinocytes / metabolism*
Membrane Proteins / genetics*
Protein Kinase C / genetics,  metabolism*
Protein Kinase C-delta / genetics,  metabolism*
Proto-Oncogene Proteins c-jun / genetics,  metabolism
RNA Interference
Transcription Factor AP-1 / genetics,  metabolism*
Reg. No./Substance:
0/Membrane Proteins; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 0/loricrin; EC 2.7.1.-/protein kinase C eta; EC Kinase C; EC Kinase C-delta

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