Document Detail

Protein kinase C alpha and gamma in N/N 1003A rabbit lens epithelial cell differentiation.
MedLine Citation:
PMID:  11239247     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine if Protein Kinase C (PKC) plays a role in the initiation of lens epithelial cell differentiation into a lens fiber cell. METHODS: PKCalpha or PKCgamma was overexpressed in N/N 1003A lens epithelial cells for up to 7 days. Phase contrast microscopy was used to observe morphological changes associated with PKCalpha or PKCgamma overexpression. Cell cycle changes in cells overexpressing PKCalpha or PKCgamma were measured using acridine orange staining and flow cytometry. Crystallin levels in cells overexpressing PKCalpha or PKCgamma were measured using Western blots and RT-PCR. RESULTS: Significant differences in cell cycling were observed between untransfected cells and those overexpressing PKCalpha or PKCgamma. Overexpression of PKCalpha and PKCgamma caused the cells to lose their epithelial-like appearance and elongate. alphaB-crystallin expression was detected in all the samples while alphaA-crystallin was detected only in cells after 7 days of PKCalpha or PKCgamma overexpression. CONCLUSIONS: The observations that alphaA-crystallin is only found in N/N 1003A cells overexpressing PKCalpha or PKCgamma for 7 days along with the finding that a block in the G0/G1 phase of the cell cycle and the consequent morphological changes are observed, indicate that PKCalpha and PKCgamma may have a role in the initiation of differentiation in lens epithelial cells.
L M Wagner; D J Takemoto
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Publication Detail:
Type:  Journal Article     Date:  2001-03-07
Journal Detail:
Title:  Molecular vision     Volume:  7     ISSN:  1090-0535     ISO Abbreviation:  Mol. Vis.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-12     Completed Date:  2001-05-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9605351     Medline TA:  Mol Vis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-62     Citation Subset:  IM    
Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.
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MeSH Terms
Acridine Orange
Blotting, Western
Cell Cycle / physiology
Cell Differentiation / physiology*
Cells, Cultured
Crystallins / metabolism
DNA Primers / chemistry
Enzyme Activation
Epithelial Cells / cytology*,  metabolism
Flow Cytometry
Gene Expression
Isoenzymes / physiology*
Lens, Crystalline / cytology*,  metabolism
Microscopy, Phase-Contrast
Protein Kinase C / physiology*
Protein Kinase C-alpha
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Crystallins; 0/DNA Primers; 0/Isoenzymes; 0/RNA, Messenger; 65-61-2/Acridine Orange; EC 2.7.1.-/protein kinase C gamma; EC Kinase C; EC Kinase C-alpha

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