Document Detail

Protein kinase C-alpha activity modulates transepithelial permeability and cell junctions in the LLC-PK1 epithelial cell line.
MedLine Citation:
PMID:  9169467     Owner:  NLM     Status:  MEDLINE    
Modulation of protein kinase C (PKC) by 12-O-tetradecanoylphorbol-13-acetate (TPA) disrupts the cell-cell junctions of the epithelial cell line LLC-PK1. To examine the role of specific PKC isoforms in this process we have created modified LLC-PK1 subclones that express wild-type and dominant negative versions of PKC-alpha under control of the tetracycline-responsive expression system. Overexpression of wild-type PKC-alpha rendered the cells more sensitive to the effects of TPA on transepithelial permeability as measured by loss of transepithelial resistance across the cell sheet. Conversely, expression of a dominant negative PKC-alpha rendered the cells more resistant to the effects of TPA as measured both by loss of transepithelial resistance as well as cell scattering. The properties of both subclones could be modulated by the addition of tetracycline, which suppressed the effect of the exogenous genes. These results indicate that the alpha isoform of PKC is at least one of the isoforms that regulate tight junctions and other cell-cell junctions of LLC-PK1 epithelia.
D Rosson; T G O'Brien; J A Kampherstein; Z Szallasi; K Bogi; P M Blumberg; J M Mullin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  272     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-06-26     Completed Date:  1997-06-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14950-3     Citation Subset:  IM    
Lankenau Medical Research Center, Wynnewood, Pennsylvania 19096-3411, USA.
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MeSH Terms
Binding Sites
Cell Membrane Permeability* / drug effects
Clone Cells
Intercellular Junctions / physiology*,  ultrastructure
Isoenzymes / biosynthesis,  metabolism*
LLC-PK1 Cells
Mutagenesis, Site-Directed
Point Mutation
Protein Kinase C / biosynthesis,  metabolism*
Protein Kinase C-alpha
Recombinant Proteins / biosynthesis,  metabolism
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
Reg. No./Substance:
0/Isoenzymes; 0/Recombinant Proteins; 16561-29-8/Tetradecanoylphorbol Acetate; 56-41-7/Alanine; 56-87-1/Lysine; EC Kinase C; EC Kinase C-alpha

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