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Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae.
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MedLine Citation:
PMID:  8294514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Potocytosis is an endocytic pathway that utilizes glycosylphosphatidylinositol-anchored membrane proteins and caveolae to concentrate and internalize small molecules. We now report that activators of protein kinase C are potent inhibitors of potocytosis. Activators such as phorbol-12-myristate-13-acetate (PMA) inhibit the internalization of receptors for 5-methyltetrahydrofolate but allow the internal receptor pool to return to the cell surface. PMA does not affect the clustering of the folate receptor but instead markedly reduces the number of caveolae. Exposure to PMA totally blocks the intracellular accumulation of 5-methyltetrahydrofolate without affecting receptor-independent uptake or the formation of polyglutamylated species of 5-methyltetrahydrofolate in the cytoplasm. These data suggest that PMA inhibits uptake by inactivating caveolae internalization.
Authors:
E J Smart; D C Foster; Y S Ying; B A Kamen; R G Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  124     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-02-25     Completed Date:  1994-02-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  307-13     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carrier Proteins / metabolism*
Cell Line
Cell Membrane / metabolism,  ultrastructure*
Endocytosis / drug effects*
Enzyme Activation
Folic Acid / metabolism*
Haplorhini
Microscopy, Electron
Protein Kinase C / metabolism*
Receptors, Cell Surface*
Tetradecanoylphorbol Acetate / metabolism,  pharmacology*
Tetrahydrofolates / metabolism*
Grant Support
ID/Acronym/Agency:
GM-43169/GM/NIGMS NIH HHS; HL-20948/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Receptors, Cell Surface; 0/Tetrahydrofolates; 0/folate-binding protein; 134-35-0/5-methyltetrahydrofolate; 16561-29-8/Tetradecanoylphorbol Acetate; 59-30-3/Folic Acid; EC 2.7.11.13/Protein Kinase C
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 2 Year: 1994
Volume: 124 Issue: 3
First Page: 307 Last Page: 313
ID: 2119940
Publisher Id: 94124626
PubMed Id: 8294514

Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae


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