Document Detail


Protein kinase C activation and vacuolation in HeLa cells invaded by Mycoplasma penetrans.
MedLine Citation:
PMID:  9788816     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The AIDS-associated Mycoplasma penetrans is capable of inducing its own uptake by non-phagocytic cells. This study investigated the invasion of HeLa cells and its consequences by confocal laser scanning microscopy. Invasion was dependent on the duration of infection and temperature, diminished by inhibiting microfilament assembly with cytochalasin D and almost completely abolished by disorganising microtubules with vinblastine or taxol. After a short infection period (< 20 min), pronounced activation of protein kinase C was detected in host cells, whereas prolonged infection resulted in intensive vacuolation of the host cells and a pronounced increment in intracellular organic peroxide levels. A marked decrease in the extent of vacuolation was observed when peroxide accumulation was partially prevented by alpha-tocopherol. The possibility that M. penetrans entry into HeLa cells involves the activation of protein kinases and the recruitment of cytoskeleton components is discussed.
Authors:
Z Borovsky; M Tarshis; P Zhang; S Rottem
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  47     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-10-29     Completed Date:  1998-10-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  915-22     Citation Subset:  IM    
Affiliation:
Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
Blotting, Western
Cell Membrane / enzymology
Cytosol / enzymology
Enzyme Activation
Fluorescent Antibody Technique
Hela Cells / enzymology,  microbiology*,  ultrastructure
Humans
Microscopy, Confocal
Microtubules / physiology
Mycoplasma penetrans / physiology*
Peroxides / metabolism
Protein Kinase C / metabolism*
Vacuoles / physiology*
Chemical
Reg. No./Substance:
0/Peroxides; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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