Document Detail

Protein kinase C activation inhibits stress-induced synthesis of heat shock protein 27 in osteoblast-like cells: function of arachidonic acid.
MedLine Citation:
PMID:  8836877     Owner:  NLM     Status:  MEDLINE    
Exposure of osteoblast-like MC3T3-E1 cells to sodium arsenite (arsenite) increased the level of heat shock protein 27 (hsp27). The effect of arsenite was dose-dependent in the range of 50 to 200 microM. Arsenite also stimulated arachidonic acid release dose-dependently in the range between 50 and 200 microM in these cells. Both indomethacin, an inhibitor of cyclooxygenase, and nordihydroguaiaretic acid, a lipoxygenase inhibitor, significantly enhanced the arsenite-induced accumulation of hsp27. Melittin, an activator of phospholipase A2, significantly enhanced the arsenite-induced accumulation of hsp27. 12-O-Tetradecanoylphorbol-13-acetate (TPA), a protein kinase C (PKC)-activating phorbol ester, inhibited the arsenite-induced accumulation of hsp27. In contrast, 4 alpha-phorbol 12,13-didecanoate (4 alpha-PDD), a PKC-nonactivating phorbol ester, had little effect. TPA suppressed the arsenite-induced arachidonic acid release, but 4 alpha-PDD had little effect. Arsenite no longer affected cAMP accumulation, inositol phosphates formation nor the formation of choline and phosphocholine in these cells. These results suggest that the response to stress of hsp27 is coupled with the metabolic activity of the arachidonic acid cascade, and the activation of PKC inhibits the induction of hsp27 through the suppression of arachidonic acid release in osteoblast-like cells.
A Suzuki; O Kozawa; Y Oiso; K Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  62     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-12-11     Completed Date:  1996-12-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  69-75     Citation Subset:  IM    
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
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MeSH Terms
3T3 Cells
Arachidonic Acid / metabolism*
Arsenites / pharmacology
Choline / biosynthesis
Cyclic AMP / metabolism
Enzyme Activation
Heat-Shock Proteins / biosynthesis*
Indomethacin / pharmacology
Inositol Phosphates / biosynthesis
Melitten / pharmacology
Nordihydroguaiaretic Acid / pharmacology
Osteoblasts / metabolism*
Phosphatidylcholines / biosynthesis
Protein Kinase C / metabolism*
Stress, Physiological / metabolism*
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Arsenites; 0/Heat-Shock Proteins; 0/Inositol Phosphates; 0/Phosphatidylcholines; 15502-74-6/arsenite; 16561-29-8/Tetradecanoylphorbol Acetate; 20449-79-0/Melitten; 500-38-9/Nordihydroguaiaretic Acid; 506-32-1/Arachidonic Acid; 53-86-1/Indomethacin; 60-92-4/Cyclic AMP; 62-49-7/Choline; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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