Document Detail

Protein kinase C activation in murine endothelioma cell lines containing middle T antigen stimulated by platelet-activating factor.
MedLine Citation:
PMID:  1525349     Owner:  NLM     Status:  MEDLINE    
Murine endothelial cell lines containing middle T antigen provide a good model to study the biology of capillary cells of different anatomical districts. These cell lines obtained from skin (sEnd.1), brain (bEnd.4), thymus (tEnd-1) and embryo tissue (eEnd.1) are activated by platelet-activating factor (PAF). PAF, but not lyso-PAF or the enantiomer (S)-PAF, induces the rapid translocation of protein kinase C (PKC) from the cytosol to the membrane. Maximal translocation of the enzyme is achieved after 5 min in all cell lines tested. The ED50 of PAF able to promote PKC translocation has a value of 5 nM in sEnd.1, bEnd.4 and eEnd.1, and 20 nM in tEnd.1, suggesting a different sensitivity between these cell lines. The data indicate that PAF activates capillary cells, thus explaining its activation of microvascular beds.
F Bussolino; A Guglielmetti; D Ghigo; G Pescarmona; A Bosia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid mediators     Volume:  5     ISSN:  0921-8319     ISO Abbreviation:  J Lipid Mediat     Publication Date:    1992 Jun-Jul
Date Detail:
Created Date:  1992-10-22     Completed Date:  1992-10-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8913460     Medline TA:  J Lipid Mediat     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  109-13     Citation Subset:  IM    
Dipartimento di Genetica, Biologia e Chimica Medica, Universita di Torino, Italy.
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MeSH Terms
Antigens, Polyomavirus Transforming
Brain Neoplasms / enzymology
Enzyme Activation
Hemangioendothelioma / immunology,  metabolism*
Platelet Activating Factor / analogs & derivatives,  pharmacology*
Protein Kinase C / metabolism*
Skin Neoplasms / enzymology
Thymus Gland / enzymology
Tumor Cells, Cultured / drug effects*
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 0/O-deacetyl platelet activating factor; 0/Platelet Activating Factor; EC Kinase C

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