Document Detail


Protein and gene expression of estrogen receptor alpha and nuclear morphology of two breast cancer cell lines after different fixation methods.
MedLine Citation:
PMID:  20025868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We assessed morphology and ERalpha protein and gene expression of two breast cancer cell lines after three different fixatives: neutral-buffered 10% formaldehyde, LN-FIX and FineFIX and varying fixation times. We found that the cell morphology was best preserved in cells fixed with LN-FIX. Two commercial fixatives used in this study shrank cells less than formalin. In immunohistochemical assay samples were stained with two different ERalpha antibodies, clone 1D5 and clone SP1. All tested fixatives were suitable for immunohistochemistry. Staining was more intensive and the number of stained cells was larger with the clone 1D5 than with the clone SP1. Our gene expression analysis showed that formalin and LN-FIX preserve the ERalpha better than FineFIX, which is advertised to be optimal for molecular analysis. Our study suggests that tissues fixed with formalin are suitable also for molecular biology assays. This makes possible to research formalin-fixed paraffin-embedded archival tissues also with molecular techniques.
Authors:
Marjukka Nyk?nen; Teijo Kuopio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-16
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  88     ISSN:  1096-0945     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-02     Completed Date:  2010-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  265-71     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
Department of Pathology, Central Finland Central Hospital, Keskussairaalantie 19, 40620 Jyv?skyl?, Finland. marjukka.nykanen@ksshp.fi
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / genetics*,  metabolism*,  pathology
Cell Culture Techniques / methods
Cell Line, Tumor
Cell Nucleus / genetics,  metabolism*
Estrogen Receptor alpha / genetics*,  metabolism*
Female
Formaldehyde
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry / methods
Polymerase Chain Reaction
RNA, Neoplasm / genetics,  isolation & purification
Reverse Transcription
Staining and Labeling / methods
Tissue Fixation / methods*
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/RNA, Neoplasm; 50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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