Document Detail


Protein feeding promotes redistribution of endogenous glucose production to the kidney and potentiates its suppression by insulin.
MedLine Citation:
PMID:  18845639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to assess in rats the effect of protein feeding on the: 1) distribution of endogenous glucose production (EGP) among gluconeogenic organs, and 2) repercussion on the insulin sensitivity of glucose metabolism. We used gene expression analyses, a combination of glucose tracer dilution and arteriovenous balance to quantify specific organ release, and hyperinsulinemic euglycemic clamps to assess EGP and glucose uptake. Protein feeding promoted a dramatic induction of the main regulatory gluconeogenic genes (glucose-6 phosphatase and phosphoenolpyruvate carboxykinase) in the kidney, but not in the liver. As a consequence, the kidney glucose release was markedly increased, compared with rats fed a normal starch diet. Protein feeding ameliorated the suppression of EGP by insulin and the sparing of glycogen storage in the liver but had no effect on glucose uptake. Combined with the previously reported induction of gluconeogenesis in the small intestine, the present work strongly suggests that a redistribution of glucose production among gluconeogenic organs might occur upon protein feeding. This phenomenon is in keeping with the improvement of insulin sensitivity of EGP, most likely involving the hepatic site. These data shed a new light on the improvement of glucose tolerance, previously observed upon increasing the amount of protein in the diet, in type 2 diabetic patients.
Authors:
Bruno Pillot; Maud Soty; Amandine Gautier-Stein; Carine Zitoun; Gilles Mithieux
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-09
Journal Detail:
Title:  Endocrinology     Volume:  150     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-29     Completed Date:  2009-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  616-24     Citation Subset:  AIM; IM    
Affiliation:
Institut National de la Santé et de la Recherche Médicale, Unité 855 and Université de Lyon, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Diet*
Drug Synergism
Eating / physiology
Fasting / metabolism,  physiology
Gene Expression Regulation / drug effects
Gluconeogenesis / drug effects,  genetics
Glucose / metabolism*
Glucose-6-Phosphatase / genetics,  metabolism
Insulin / pharmacology*
Kidney / drug effects*,  metabolism
Liver / drug effects,  metabolism
Male
Protein-Serine-Threonine Kinases / genetics,  metabolism
Proteins / pharmacology*
Rats
Rats, Sprague-Dawley
Tissue Distribution / drug effects
Chemical
Reg. No./Substance:
0/Proteins; 11061-68-0/Insulin; 50-99-7/Glucose; EC 2.7.1.-/phosphoenolpyruvate carboxylase kinase; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 3.1.3.9/Glucose-6-Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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