| Protein extract of ultraviolet-irradiated human skin keratinocytes promote the expression of mitogen-activated protein kinases, nuclear factor-κB and interferon regulatory factor-3 in Langerhans cells via Toll-like receptor 2 and 4. | |
| | |
MedLine Citation:
|
PMID: 23281696 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
AIM: In this study, we investigated whether the protein extract of ultraviolet-irradiated human skin keratinocytes can activate Toll-like receptor 2 and Toll-like receptor 4 of Langerhans cells and induce the downstream gene expression of mitogen-activated protein kinases, nuclear factor-κB and interferon regulatory factor-3. METHODS: The protein expression of mitogen-activated protein kinases, nuclear factor-κB and interferon regulatory factor-3 in Langerhans cells and the protein expression of HSP60, HSP70 and β-defensin 2 in keratinocytes were examined using Western blot analysis. Langerhans cells were pretreated with or without Toll-like receptor 2 and Toll-like receptor 4 siRNA. RESULTS: We found that the protein extract of ultraviolet-irradiated keratinocytes upregulated the expression of mitogen-activated protein kinases, nuclear factor-κB and interferon regulatory factor-3 in Langerhans cells via Toll-like receptor 2 and Toll-like receptor 4. We also found that ultraviolet radiation upregulated the expression HSP60, HSP70 and β-defensin 2 in keratinocytes. CONCLUSIONS: Our previous study demonstrated that ultraviolet radiation upregulated Toll-like receptor 2 and Toll-like receptor 4 expression in Langerhans cells. Ultraviolet radiation also upregulated mitogen-activated protein kinases and nuclear factor-κB/p65 expression via Toll-like receptor 2 and Toll-like receptor 4, and upregulated interferon regulatory factor-3 expression partially via Toll-like receptor 4. So we conclude that ultraviolet radiation can directly or indirectly activate keratinocytes to induce endogenous ligands which stimulate Toll-like receptor 2- or Toll-like receptor 4-dependent signaling cascade in Langerhans cells, sequentially influence innate and adaptive immune responses. |
| | |
Authors:
|
Xiao-Yong Wang; Cheng-Jun Tao; Qiao-Yun Wu; Cheng-Da Yuan |
Related Documents
:
|
20950606 - Pharmacological profile of as1670542, a novel orally-active human thrombopoietin recept... 1846866 - Effect of receptor kinase inactivation on the rate of internalization and degradation o... 2850496 - Cdna cloning and expression of a human platelet-derived growth factor (pdgf) receptor s... 17351626 - Molecular imaging of vegf receptors in angiogenic vasculature with single-chain vegf-ba... 17920266 - Structure-activity relationship study on the 6-membered heteroaromatic ring system of d... 10908156 - Interaction of the receptor for advanced glycation end products (rage) with transthyret... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Photodermatology, photoimmunology & photomedicine Volume: 29 ISSN: 1600-0781 ISO Abbreviation: Photodermatol Photoimmunol Photomed Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-01-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9013641 Medline TA: Photodermatol Photoimmunol Photomed Country: England |
Other Details:
|
Languages: eng Pagination: 41-8 Citation Subset: IM |
Copyright Information:
|
© 2013 John Wiley & Sons A/S. |
Affiliation:
|
Department of Dermatology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of serum 25-hydroxyvitamin D levels in vitiligo patients with and without autoimmune dise...
Next Document: Second-degree burns caused by exposure to sunbed with displaced filter in the facial tanner: a repor...