Document Detail


Protein expressions in macrophage-derived foam cells: comparative analysis by two-dimensional gel electrophoresis.
MedLine Citation:
PMID:  12956934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the overall protein expression changes induced by oxidized low-density lipoprotein (ox-LDL) in the U937 foam cells. METHODS: Foam cell model was established by incubating the human monoblastic leukemia (U937) cells with ox-LDL. Each protein samples in U937 control cells and U937 foam cells were separated by two-dimensional gel electrophoresis (2-DE). After the gels were stained by silver staining method, the images were analyzed by PDquest 2D-image-analysis software (Bio-Rad). Some of the spots were available via the Internet with links to the U937 proteomic map provided from the ExPASy Proteomics server. RESULTS: Using 2-DE, the overall protein map was obtained, in which 150 spots were matched with the control gel (match ratio: 75 %). Compared with U937 cells, 37 spots significantly changed in the foam cells (P<0.05), among which the expression levels in 28 spots increased and those in 9 spots decreased. Especially, 8 spots in U937 cells were absent in the foam cells, while 11 spots in the foam cells were absent in the control cells. CONCLUSION: The changed protein profiles induced by ox-LDL in U937 foam cells were established to support the functional studies on the macrophage-derived foam cells in atherosclerotic pathological states.
Authors:
Yan-Ling Yu; Peng-Yuan Yang; Hui-Zhi Fan; Zhen-Yu Huang; Yao-Cheng Rui; Peng-Yuan Yang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  24     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-05     Completed Date:  2004-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  873-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Fudan University, Shanghai 200433, China.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Electrophoresis, Gel, Two-Dimensional / methods*
Foam Cells / metabolism
Humans
Lipoproteins, LDL / pharmacology*
Protein Biosynthesis
Proteins / analysis*
U937 Cells
Chemical
Reg. No./Substance:
0/Lipoproteins, LDL; 0/Proteins; 0/oxidized low density lipoprotein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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