| Protein expression profile in the striatum of acute methamphetamine-treated rats. | |
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MedLine Citation:
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PMID: 16729985 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Methamphetamine (MAP) is an addictive drug with psychostimulant effects. It is known that MAP induces behavioral changes, including hyperlocomotion and stereotypical movements in rodents. These behavioral changes induced by MAP have been compared with behavioral changes in patients with MAP addiction and MAP psychosis. However, little is known about the underlying mechanisms of MAPs effects on global protein expression. 2-DE proteomics allows us to examine global changes in protein expression in complex biological systems and to propose possible hypotheses of the underlying mechanisms in various pathological conditions. In the present study, we aim to identify protein expression profiles in the striatum (ST) of acute low dose MAP (1 mg/kg)-treated rats using 2-DE proteomics. MATERIALS AND METHODS: Rats were given an intraperitoneal injection of MAP (1 mg/kg) or saline. Locomotor activity was monitored. Proteins were extracted from the ST of MAP-treated and saline-treated control rats then separated and analyzed using 2-DE. RESULTS. Low dose MAP administration significantly increased locomotor activity. 2-DE analysis revealed 36 protein spots differentially regulated in the ST of acute MAP-treated rats compared to a vehicle-treated control. 26 protein spots have been identified using MALDI-TOF, including phosphoglycerate kinase 1, Dihydrolipoamide dehydrogenase, Voltage-dependent anion-selective channel protein 1, Rho GDP dissociation inhibitor alpha, peroxiredoxin 2, ubiquitin carboxy-terminal hydrolase L1, and actin beta, N-tropomodulin. DISCUSSION: These proteins could be related to underlying mechanisms of acute low dose MAP effects, indicating mitochondrial dysfunction, oxidative damages, lysosomal degradation, degenerative processes, and neuronal modification. |
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Authors:
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Takeshi Iwazaki; Iain S McGregor; Izuru Matsumoto |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-26 |
Journal Detail:
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Title: Brain research Volume: 1097 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-07-10 Completed Date: 2006-10-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 19-25 Citation Subset: IM |
Affiliation:
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Discipline of Pathology, The University of Sydney, NSW 2006, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Corpus Striatum / drug effects*, metabolism* Gene Expression Profiling / methods*, statistics & numerical data Male Methamphetamine / administration & dosage* Motor Activity / drug effects, physiology Nerve Tissue Proteins / biosynthesis*, genetics* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 537-46-2/Methamphetamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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