| Protein expression in relation to the cell cycle of exponentially growing human prostatic epithelial cells. | |
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MedLine Citation:
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PMID: 2039807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This report concerns the study of the relationship between protein expression and the cell cycle in exponentially proliferating benign and malignant human prostate epithelial cells in short-term cultures. Multiparameter flow cytometric measurements were performed to correlate the expression of prostate-specific acid phosphatase, epithelial membrane antigen and epitectin with cell cycle progression. The expression of the three proteins was heterogeneous in G1 cells. The early post-mitotic cells exhibited the lowest levels when compared with late G1 cells, wherein the expression was many times greater. There was no further increase as the cells progressed through S and G2 + M. These findings, corroborating prior observations in other systems, suggest the possibility that the levels of the proteins studied increase during the G1 phase of the cell cycle and drop during or immediately after cytokinesis. As an alternate explanation, the heterogeneity of protein expression characteristic of G1 cells may be due, at least in part, to an asymmetric apportionment of cell constituents at mitosis. |
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Authors:
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F Herz; B Czerniak; D Deitch; R P Wersto; D A Simmons; L G Koss |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell proliferation Volume: 24 ISSN: 0960-7722 ISO Abbreviation: Cell Prolif. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-07-11 Completed Date: 1991-07-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9105195 Medline TA: Cell Prolif Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 321-30 Citation Subset: IM |
Affiliation:
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Department of Pathology, Montefiore Medical Center, Bronx, NY 10467-2401. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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biosynthesis Antigens, Tumor-Associated, Carbohydrate / biosynthesis Cell Cycle Cell Division Cells, Cultured Epithelial Cells Epithelium / metabolism Flow Cytometry Fluorescent Antibody Technique Humans Kinetics Male Membrane Glycoproteins / biosynthesis Mucin-1 Prostate / cytology*, metabolism Prostatic Neoplasms Protein Biosynthesis* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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5R01 CA34790/CA/NCI NIH HHS; 5R01 CA47512/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Tumor-Associated, Carbohydrate; 0/Membrane Glycoproteins; 0/Mucin-1; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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