Document Detail

Protein expression in relation to the cell cycle of exponentially growing human prostatic epithelial cells.
MedLine Citation:
PMID:  2039807     Owner:  NLM     Status:  MEDLINE    
This report concerns the study of the relationship between protein expression and the cell cycle in exponentially proliferating benign and malignant human prostate epithelial cells in short-term cultures. Multiparameter flow cytometric measurements were performed to correlate the expression of prostate-specific acid phosphatase, epithelial membrane antigen and epitectin with cell cycle progression. The expression of the three proteins was heterogeneous in G1 cells. The early post-mitotic cells exhibited the lowest levels when compared with late G1 cells, wherein the expression was many times greater. There was no further increase as the cells progressed through S and G2 + M. These findings, corroborating prior observations in other systems, suggest the possibility that the levels of the proteins studied increase during the G1 phase of the cell cycle and drop during or immediately after cytokinesis. As an alternate explanation, the heterogeneity of protein expression characteristic of G1 cells may be due, at least in part, to an asymmetric apportionment of cell constituents at mitosis.
F Herz; B Czerniak; D Deitch; R P Wersto; D A Simmons; L G Koss
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell proliferation     Volume:  24     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-07-11     Completed Date:  1991-07-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  321-30     Citation Subset:  IM    
Department of Pathology, Montefiore Medical Center, Bronx, NY 10467-2401.
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MeSH Terms
Acid Phosphatase / biosynthesis
Antigens, Tumor-Associated, Carbohydrate / biosynthesis
Cell Cycle
Cell Division
Cells, Cultured
Epithelial Cells
Epithelium / metabolism
Flow Cytometry
Fluorescent Antibody Technique
Membrane Glycoproteins / biosynthesis
Prostate / cytology*,  metabolism
Prostatic Neoplasms
Protein Biosynthesis*
Tumor Cells, Cultured
Grant Support
5R01 CA34790/CA/NCI NIH HHS; 5R01 CA47512/CA/NCI NIH HHS
Reg. No./Substance:
0/Antigens, Tumor-Associated, Carbohydrate; 0/Membrane Glycoproteins; 0/Mucin-1; EC Phosphatase

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