Document Detail


Protein expression in orthocortical and paracortical cells of merino wool fibers.
MedLine Citation:
PMID:  19292463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crimp and bulk, important wool fiber properties, are thought to be related to differences in the protein composition of the orthocortex and paracortex. Fiber morphological studies have demonstrated that the paracortex has a higher proportion of matrix and cysteine than the orthocortex. While there is some evidence for the differential expression of genes between these cell types in the follicle, this has not been demonstrated satisfactorily in the mature fiber. Using proteolytic digestion of wool fibers, followed by ultrasonic disruption to obtain relatively pure fractions of both cell types, the KAP3 high sulfur protein family was found to be present in higher concentrations in the paracortex. This significant finding provides an explanation for the higher cysteine content reported in the paracortex. This represents an advance in our understanding of protein expression variation in the orthocortex and paracortex, and how this relates to key physical and mechanical properties of wool fibers.
Authors:
Jeffrey E Plowman; Santanu Deb-Choudhury; Warren G Bryson; Stefan Clerens; Jolon M Dyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-18     Completed Date:  2009-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2174-80     Citation Subset:  IM    
Affiliation:
AgResearch Ltd, Private Bag, Christchurch, New Zealand. jeff.plowman@agresearch.co.nz
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Count
Cell Separation
Gene Expression*
Intermediate Filament Proteins / analysis,  chemistry,  genetics
Molecular Sequence Data
Physicochemical Phenomena
Proteins / analysis*,  chemistry,  genetics
Sheep*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Wool / chemistry*,  cytology*
Chemical
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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