| Protein expression by planktonic and biofilm cells of Streptococcus mutans. | |
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MedLine Citation:
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PMID: 11728728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Streptococcus mutans, a major causal agent of dental caries, functions in nature as a component of a biofilm on teeth (dental plaque) and yet very little information is available on the physiology of the organism in such surface-associated communities. As a consequence, we undertook to examine the synthesis of proteins by planktonic and biofilm cells growing in a biofilm chemostat at pH 7.5 at a dilution rate of 0.1 h(-1) (mean generation time=7 h). Cells were incubated with (14)C-labelled amino acids, the proteins extracted and separated by two-dimensional electrophoresis followed by autoradiography and computer-assisted image analysis. Of 694 proteins analysed, 57 proteins were enhanced 1.3-fold or greater in biofilm cells compared to planktonic cells with 13 only expressed in sessile cells. Diminished protein expression was observed with 78 proteins, nine of which were not expressed in biofilm cells. The identification of enhanced and diminished proteins by mass spectrometry and computer-assisted protein sequence analysis revealed that, in general, glycolytic enzymes involved in acid formation were repressed in biofilm cells, while biosynthetic processes were enhanced. The results show that biofilm cells possess novel proteins, of as yet unknown function, that are not present in planktonic cells. |
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Authors:
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G Svensäter; J Welin; J C Wilkins; D Beighton; I R Hamilton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology letters Volume: 205 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-30 Completed Date: 2002-02-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 139-46 Citation Subset: IM |
Affiliation:
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Department of Oral Biology, Centre for Oral Health Sciences, Malmö University, Malmö, Sweden. gunnel.svensater@od.mah.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Proteins / chemistry, metabolism* Biofilms / growth & development* Culture Media Dental Caries / microbiology Electrophoresis, Gel, Two-Dimensional Humans Image Processing, Computer-Assisted Plankton / metabolism* Streptococcus mutans / growth & development*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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