Document Detail

Protein dye affinity chromatography using immobilized tetraiodofluorescein.
MedLine Citation:
PMID:  6270140     Owner:  NLM     Status:  MEDLINE    
The chromatographic behavior of a heterogeneous protein mixture and of a series of homogeneous proteins on the immobilized dye tetraiodofluorescein has been observed and analyzed. Less than 6%, of the millimolar concentration of dye immobilized to a porous agarose matrix is accessible to protein. The affinity of a protein for immobilized dye is dramatically increased by insertion of apolar spacer atoms between the dye and the matrix. Dye columns constructed with a 9-atom spacer can be used to advantage for the retention and competitive elution of proteins not found previously amenable to dye chromatography.
R F Tucker; J Babul; E Stellwagen
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  256     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1981-12-21     Completed Date:  1981-12-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10993-8     Citation Subset:  IM    
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MeSH Terms
Chromatography, Affinity / methods
Coloring Agents
Proteins / isolation & purification*
Structure-Activity Relationship
Grant Support
Reg. No./Substance:
0/Coloring Agents; 0/Fluoresceins; 0/Ligands; 0/Proteins; 16423-68-0/Erythrosine

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