| Protein disulfide isomerase is cleaved by caspase-3 and -7 during apoptosis. | |
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MedLine Citation:
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PMID: 17978580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptotic signals are typically accompanied by activation of aspartate-specific cysteine proteases called caspases, and caspase-3 and -7 play crucial roles in the execution of apoptosis. Previously, using the proteomic approach, protein disulfide isomerase (PDI) was found to be a candidate substrate of caspase-7. This abundant 55 kDa protein introduces disulfide bonds into proteins (via its oxidase activity) and catalyzes the rearrangement of incorrect disulfide bonds (via its isomerase activity). PDI is abundant in the ER but is also found in non-ER locations. In this study we demonstrated that PDI is cleaved by caspase-3 and -7 in vitro. In addition, in vivo experiment showed that it is cleaved during etoposide-induced apoptosis in HL-60 cells. Subcellular fractionation showed that PDI was also present in the cytosol. Furthermore, only cytosolic PDI was clearly digested by caspase-3 and -7. It was also confirmed by confocal image analysis that PDI and caspase-7 partially co-localize in both resting and apoptotic MCF-7 cells. Overexpression of cytosolic PDI (ER retention sequence deleted) inhibited cell death after an apoptotic stimulus. These data indicate that cytosolic PDI is a substrate of caspase-3 and -7, and that it has an anti-apoptotic action. |
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Authors:
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Kyung Sook Na; Byoung Chul Park; Mi Jang; Sayeon Cho; Do Hee Lee; Sunghyun Kang; Chong-Kil Lee; Kwang-Hee Bae; Sung Goo Park |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecules and cells Volume: 24 ISSN: 1016-8478 ISO Abbreviation: Mol. Cells Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-11-05 Completed Date: 2007-12-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9610936 Medline TA: Mol Cells Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 261-7 Citation Subset: IM |
Affiliation:
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Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis*
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drug effects Caspase 3 / metabolism* Caspase 7 / metabolism* Cytosol / drug effects, enzymology Etoposide / pharmacology HL-60 Cells Humans Microscopy, Confocal Protein Disulfide-Isomerases / metabolism* Protein Transport / drug effects Recombinant Proteins / metabolism Subcellular Fractions / enzymology Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 33419-42-0/Etoposide; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7; EC 5.3.4.1/Protein Disulfide-Isomerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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