Document Detail


Protein deprivation attenuates Hsp expression in fat tissue.
MedLine Citation:
PMID:  22081321     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For ruminants, dietary protein is the first limiting component to the utilization of low-quality forage. Throughout gestation, low-protein intake may result in prenatal programming that causes various metabolic disturbances and physiological modulations to dams and their developing embryos. We studied the effect of long-term low-protein diet (LPD) on physiological, biochemical, and molecular parameters of the energy status in gestating beef cows. LPD resulted in significant reductions in feed intake and heart rate and promoted a negative retained energy status already after 3 weeks. Elevated levels of plasma creatinine and non-esterified fatty acids indicate endogenous degradation of fat and protein as a response to the demands in energy and nitrogen. Increasing levels of β-hydroxybutyrate confirmed the negative energy status obtained by the physiological measurements. At the molecular level, subcutaneous fat, Hsp90, Hsp70, and proteasome subunits decreased significantly after 3 months on LPD, in parallel with an increase of adipocyte fatty acid-binding protein. These results may indicate a decrease in turn-over of proteins, at the cost of induced lipolysis, and suggest that the response to protein deprivation, when examined in an energy-storing tissue, includes downregulation of the constitutive heat shock proteins involved in the protein degradation pathway of energy production and upregulation of tissue-specific genes such as those involved in energy production from fat degradation.
Authors:
Harel Eitam; Rotem Agmon; Aviv Asher; Arieh Brosh; Alla Orlov; Ido Izhaki; Ariel Shabtay
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-12
Journal Detail:
Title:  Cell stress & chaperones     Volume:  17     ISSN:  1466-1268     ISO Abbreviation:  Cell Stress Chaperones     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-07-20     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9610925     Medline TA:  Cell Stress Chaperones     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  339-47     Citation Subset:  IM    
Affiliation:
Institute of Animal Science, Department of Ruminant Science, Newe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay, Israel.
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxybutyric Acid / blood
Adipose Tissue / metabolism*
Animals
Cattle
Creatinine / blood
Diet, Protein-Restricted*
Energy Metabolism
Fatty Acid-Binding Proteins / metabolism
Fatty Acids, Nonesterified / blood
Female
Heart Rate / physiology
Heat-Shock Proteins / metabolism*
Lipolysis
Proteasome Endopeptidase Complex / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acid-Binding Proteins; 0/Fatty Acids, Nonesterified; 0/Heat-Shock Proteins; 300-85-6/3-Hydroxybutyric Acid; 60-27-5/Creatinine; EC 3.4.25.1/Proteasome Endopeptidase Complex
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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