| Protein degradation during renal passage in normal kidneys is inhibited in experimental albuminuria. | |
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MedLine Citation:
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PMID: 9279205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. Tritium labelled proteins, namely bovine serum albumin ([3H]BSA), rat serum albumin ([3H]RSA), anionic horseradish peroxidase ([3H]aHRP) and immunoglobulin present in urine fractions from rat filtration studies in vivo and isolated perfused rat kidneys (IPKs) have been shown by gel chromatographic analysis to be severely degraded to small peptides. The degradation of RSA and BSA in vivo has been shown to be similar. 2. Degradation of proteins in the urine from IPK experiments was inhibited by including 150 mmol/l lysine in the perfusate. Similarly, [3H]BSA and [3H]aHRP excreted from rats with puromycin aminonucleoside nephrosis was again essentially intact for both IPK and in vivo experiments. 3. It appears that the degradation of proteins observed in urine obtained from control kidneys is due, in part, to proteolytic activity associated with the proximal tubule. Inhibition of proximal tubule function, which occurs for both lysine and puromycin aminonucleoside treatments (as calibrated by lysozyme uptake), results in inhibition of the degradation observed. Glomerular epithelial cells could also contribute to the degradation. 4. There was no generation of low-molecular-weight material in the perfusate or plasma arising from breakdown of circulating proteins or recycling of potential degradation products from the tubules. |
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Authors:
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T M Osicka; W D Comper |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical science (London, England : 1979) Volume: 93 ISSN: 0143-5221 ISO Abbreviation: Clin. Sci. Publication Date: 1997 Jul |
Date Detail:
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Created Date: 1997-09-09 Completed Date: 1997-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905731 Medline TA: Clin Sci (Lond) Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 65-72 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albuminuria
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metabolism*,
urine Animals Antimetabolites Chromatography Horseradish Peroxidase / metabolism Immunoglobulins / metabolism Kidney / metabolism* Male Molecular Weight Nephrosis / metabolism Perfusion Proteins / metabolism* Puromycin Aminonucleoside Rats Rats, Sprague-Dawley Serum Albumin / metabolism Serum Albumin, Bovine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites; 0/Immunoglobulins; 0/Proteins; 0/Serum Albumin; 0/Serum Albumin, Bovine; 58-60-6/Puromycin Aminonucleoside; EC 1.11.1.-/Horseradish Peroxidase |
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