Document Detail


Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor-induced neurite outgrowth in PC12 cells.
MedLine Citation:
PMID:  20434449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SIRT1, a NAD(+)-dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)-induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1-siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF-dependent neurite outgrowth, and a cytoplasmic dominant-negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF-induced neurite outgrowth of PC12 cells.
Authors:
Toshiya Sugino; Mitsuhisa Maruyama; Masaya Tanno; Atsushi Kuno; Kiyohiro Houkin; Yoshiyuki Horio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-29
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-07-05     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2821-6     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Affiliation:
Department of Pharmacology, Sapporo Medical University, Chuo-ku, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / drug effects,  genetics
Cytoplasm / metabolism*
Immunoblotting
Immunohistochemistry
NAD / pharmacology
Nerve Growth Factors / pharmacology*
Neurites / drug effects*,  metabolism*
PC12 Cells
Rats
Sirtuin 1 / genetics,  metabolism*
Stilbenes / pharmacology
Chemical
Reg. No./Substance:
0/Nerve Growth Factors; 0/Stilbenes; 53-84-9/NAD; EC 3.5.1.-/Sirt1 protein, rat; EC 3.5.1.-/Sirtuin 1; Q369O8926L/resveratrol

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