Document Detail

Protein composition of the chromosomal scaffold and interphase nuclear matrix.
MedLine Citation:
PMID:  3987439     Owner:  NLM     Status:  MEDLINE    
Residual protein structures were prepared from isolated chromosomes and interphase nuclei of in vitro cultured bovine liver cells and the protein compositions were analysed. Chromosomes with minimal cytoplasmic contamination were obtained by a simple procedure using a pH 8 isolation medium containing Triton X-100 and polyamines, and residual protein-DNA complexes were prepared by extraction with 2 M NaCl. Residual protein structures were also obtained by digesting isolated chromosomes with staphylococcal nuclease. Protein compositions of both structures as obtained by SDS-polyacrylamide gel electrophoresis were essentially the same. Residual protein structures were prepared from isolated nuclei by the same procedures. The major nuclear matrix proteins, i.e., the lamins A, B, and C, were not found in the chromosomes and chromosome scaffolds. On the other hand, the residual chromosome structures contained two major polypeptides of 37 and 83 kilodalton relative molecular weights that were absent from the nuclear matrix preparations. A few polypeptides with the same or very similar electrophoretic mobilities were found in the residual structures of both the nuclei and the chromosomes.
A C Pieck; H M van der Velden; A A Rijken; J M Neis; F Wanka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chromosoma     Volume:  91     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1985  
Date Detail:
Created Date:  1985-05-29     Completed Date:  1985-05-29     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  137-44     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / analysis,  ultrastructure*
Cells, Cultured
Chromosomal Proteins, Non-Histone / analysis*
Nucleoproteins / analysis*
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/Lamins; 0/Nucleoproteins

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