| Protein binding and in vitro serum thromboxane B2 inhibition by flunixin meglumine and meclofenamic acid in dog, goat and horse blood. | |
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MedLine Citation:
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PMID: 8819199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Flunixin was highly protein bound in the serum of dogs (92.2 per cent), goats (84.8 per cent) and horses (86.9 per cent). Meclofenamic acid was also highly protein bound, although there were larger differences between the extent of the binding in dogs (90.3 per cent), goats (84.7 per cent) and horses (99.8 per cent). Both flunixin and meclofenamic acid were potent inhibitors of the in vitro generation of thromboxane (Tx) B2 in blood. Flunixin inhibited the generation of TxB2 by 50 per cent of the maximum response (IC50) in dog, goat and horse blood at concentrations of 0.10, 0.02 and 0.04 microM respectively and by 100 per cent (Imax) at 2.07, 0.14 and 2.07 microM respectively. The IC50 values of meclofenamic acid in dogs, goats and horses were 0.77, 0.80 and 0.30 microM respectively and the Imax values were 3.93, 3.63 and 3.56 microM respectively. When the concentrations of flunixin were corrected for protein binding, it was estimated that the IC50 of the unbound fractions in dogs, goats and horses were 0.008, 0.003 and 0.005 microM, respectively. Similarly corrected values for meclofenamic acid were 0.075, 0.122 and 0.001 microM respectively. |
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Authors:
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E A Galbraith; Q A McKellar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Research in veterinary science Volume: 61 ISSN: 0034-5288 ISO Abbreviation: Res. Vet. Sci. Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1996-12-05 Completed Date: 1996-12-05 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0401300 Medline TA: Res Vet Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 78-81 Citation Subset: IM |
Affiliation:
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Department of Veterinary Pharmacology, University of Glasgow Veterinary School. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents, Non-Steroidal / blood, pharmacology* Blood Proteins / drug effects, metabolism* Clonixin / analogs & derivatives*, blood, pharmacology Dogs Goats Horses Kinetics Meclofenamic Acid / blood, pharmacology* Protein Binding Thromboxane B2 / biosynthesis, blood* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Blood Proteins; 17737-65-4/Clonixin; 42461-84-7/flunixin meglumine; 54397-85-2/Thromboxane B2; 644-62-2/Meclofenamic Acid |
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