Document Detail

Protein binding and in vitro serum thromboxane B2 inhibition by flunixin meglumine and meclofenamic acid in dog, goat and horse blood.
MedLine Citation:
PMID:  8819199     Owner:  NLM     Status:  MEDLINE    
Flunixin was highly protein bound in the serum of dogs (92.2 per cent), goats (84.8 per cent) and horses (86.9 per cent). Meclofenamic acid was also highly protein bound, although there were larger differences between the extent of the binding in dogs (90.3 per cent), goats (84.7 per cent) and horses (99.8 per cent). Both flunixin and meclofenamic acid were potent inhibitors of the in vitro generation of thromboxane (Tx) B2 in blood. Flunixin inhibited the generation of TxB2 by 50 per cent of the maximum response (IC50) in dog, goat and horse blood at concentrations of 0.10, 0.02 and 0.04 microM respectively and by 100 per cent (Imax) at 2.07, 0.14 and 2.07 microM respectively. The IC50 values of meclofenamic acid in dogs, goats and horses were 0.77, 0.80 and 0.30 microM respectively and the Imax values were 3.93, 3.63 and 3.56 microM respectively. When the concentrations of flunixin were corrected for protein binding, it was estimated that the IC50 of the unbound fractions in dogs, goats and horses were 0.008, 0.003 and 0.005 microM, respectively. Similarly corrected values for meclofenamic acid were 0.075, 0.122 and 0.001 microM respectively.
E A Galbraith; Q A McKellar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Research in veterinary science     Volume:  61     ISSN:  0034-5288     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-12-05     Completed Date:  1996-12-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  78-81     Citation Subset:  IM    
Department of Veterinary Pharmacology, University of Glasgow Veterinary School.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / blood,  pharmacology*
Blood Proteins / drug effects,  metabolism*
Clonixin / analogs & derivatives*,  blood,  pharmacology
Meclofenamic Acid / blood,  pharmacology*
Protein Binding
Thromboxane B2 / biosynthesis,  blood*
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Blood Proteins; 17737-65-4/Clonixin; 42461-84-7/flunixin meglumine; 54397-85-2/Thromboxane B2; 644-62-2/Meclofenamic Acid

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