Document Detail


Protein-based matrix interferences in ligand-binding assays.
MedLine Citation:
PMID:  24830897     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
An adequate bioanalytical support for a typical biotherapeutic requires a number of assays, including those to measure drug concentration and to assess induction of specific immune responses. Ligand-binding assays are the most commonly used platform in bioanalysis of biotherapeutics. Ligand-binding assays are frequently designed to detect appropriate analytes in complex biological matrices with limited or no sample pretreatment steps. The complex composition of the test matrix is highly diverse and varies from normal to disease populations. Additional post-treatment changes are often observed, including induction of antidrug antibodies. Due to potential interaction of biological matrix components, for example, rheumatoid factors, heterophilic antibodies and human anti-animal antibodies, with the test analyte or assay reagents, ligand-binding assays are often subjected to various degrees of matrix interferences that lead to an erroneous under- or over-reporting of the analyte concentration. Impact of various matrix components and practical means designed to mitigate interferences are discussed in this Review.
Authors:
Boris Gorovits; Jim McNally; Corinna Fiorotti; Sheldon Leung
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  6     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-05-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  1131-40     Citation Subset:  IM    
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