| Protein arrays based on biotin-streptavidin system for the simultaneous detection of TORCH infections. | |
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MedLine Citation:
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PMID: 18572639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper, we developed a protein array based on biotin-streptavidin system (PABS) used in the identification of IgM antibodies against TORCH antigens, including toxoplasma gondii (TOX), rubella virus (RuV), cytomegalovirus (CMV) and herpes simplex virus types II (HSV-2) antigens. The detection signal intensities and sensitivities between the PABS and the direct labeling array system (DLAS) were compared. The linear ranges of detectable IgM antibodies in PABS were 0.485-1000 microg/mL, which was more sensitive than DLAS. Quantitatively, the lowest detectable amount for IgM antibodies on each spot of the PABS was 0.25 pg. Furthermore, sixty serum samples from patients were tested with the PABS in TORCH detection. All the results were correspondingly confirmed with ELISA assay. No significant differences in identifying TORCH specific IgM antibodies were found between the PABS and ELISA assay. There was a good concordance between PABS and ELISA in the classification of sera. The results suggested that the PABS was more sensitive, sample-saving and suitable for multi-pathogens parallel clinical detection. |
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Authors:
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Li Jiang; Zhangbin Yu; Zuming Tang; Tao Jiang; Chunxiu Zhang; Zuhong Lu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of nanoscience and nanotechnology Volume: 8 ISSN: 1533-4880 ISO Abbreviation: J Nanosci Nanotechnol Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-06-24 Completed Date: 2008-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088195 Medline TA: J Nanosci Nanotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 2286-92 Citation Subset: IM |
Affiliation:
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School of Clinical Medicine, Southeast University, Nanjing 210009, P. R. China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotin
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chemistry* Cytomegalovirus Infections / diagnosis* Enzyme-Linked Immunosorbent Assay Herpes Simplex / diagnosis* Humans Protein Array Analysis* Reagent Kits, Diagnostic Rubella / diagnosis* Sensitivity and Specificity Streptavidin / chemistry* Toxoplasmosis / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/Reagent Kits, Diagnostic; 58-85-5/Biotin; 9013-20-1/Streptavidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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