| Protein adsorption and cell adhesion on nanoscale bioactive coatings formed from poly(ethylene glycol) and albumin microgels. | |
| | |
MedLine Citation:
|
PMID: 18771802 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Late-term thrombosis on drug-eluting stents is an emerging problem that might be addressed using extremely thin, biologically active hydrogel coatings. We report a dip-coating strategy to covalently link poly(ethylene glycol) (PEG) to substrates, producing coatings with approximately <100 nm thickness. Gelation of PEG-octavinylsulfone with amines in either bovine serum albumin (BSA) or PEG-octaamine was monitored by dynamic light scattering (DLS), revealing the presence of microgels before macrogelation. NMR also revealed extremely high end-group conversions prior to macrogelation, consistent with the formation of highly crosslinked microgels and deviation from Flory-Stockmayer theory. Before macrogelation, the reacting solutions were diluted and incubated with nucleophile-functionalized surfaces. Using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance with dissipation (QCM-D), we identified a highly hydrated, protein-resistant layer with a thickness of approximately 75 nm. Atomic force microscopy in buffered water revealed the presence of coalesced spheres of various sizes but with diameters less than about 100 nm. Microgel-coated glass or poly(ethylene terephthalate) exhibited reduced protein adsorption and cell adhesion. Cellular interactions with the surface could be controlled by using different proteins to cap unreacted vinylsulfone groups within the coating. |
| | |
Authors:
|
Evan A Scott; Michael D Nichols; Lee H Cordova; Brandon J George; Young-Shin Jun; Donald L Elbert |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-09-03 |
Journal Detail:
|
Title: Biomaterials Volume: 29 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2008 Dec |
Date Detail:
|
Created Date: 2008-09-23 Completed Date: 2008-12-30 Revised Date: 2010-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
|
Languages: eng Pagination: 4481-93 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Engineering and Center for Materials Innovation, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, MO 63105, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adsorption Animals CHO Cells Cell Adhesion Cell Line Coated Materials, Biocompatible / chemistry* Cricetinae Cricetulus Fibrinogen / metabolism Fibroblasts / metabolism Gels Glass / chemistry Humans Mice Polyethylene Glycols / chemistry* Protein Binding Proteins / metabolism* Serum Albumin, Bovine / chemistry* Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HL085364-02/HL/NHLBI NIH HHS; R01HL085364/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Coated Materials, Biocompatible; 0/Gels; 0/Polyethylene Glycols; 0/Proteins; 0/Serum Albumin, Bovine; 9001-32-5/Fibrinogen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Say it with flowers! An fMRI study of object mediated communication.
Next Document: Detection of the relevant type of locomotion in infancy: Crawlers versus walkers.