Document Detail


Protein adsorption on biodegradable polyanhydride microparticles.
MedLine Citation:
PMID:  20740599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vitro adsorption of plasma proteins on polyanhydride microparticles based on sebacic acid (SA), 1,6-bis(p-carboxyphenoxy)hexane (CPH), and 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) was studied. Three model proteins from bovine serum (albumin (BSA), immunoglobulin G (IgG), and fibrinogen (Fg)) were used. The adsorption was studied using X-Ray Photoelectron Spectroscopy and gel electrophoresis. 2D electrophoresis was used to study the adsorption of plasma proteins from bovine serum. Differences in the amount of protein adsorbed were detected as a function of the following: (i) copolymer composition and (ii) specific protein studied. A direct correlation between polymer hydrophobicity and protein adsorbed was observed and higher quantities of Fg and IgG were absorbed. In vitro release studies were performed with ovalbumin-encapsulated microparticles that were incubated with Fg; these studies showed a reduction in the amount of ovalbumin released from the microparticles when Fg is adsorbed on the surface. An understanding of protein adsorption patterns on parenteral delivery devices is valuable in optimizing their in vivo performance.
Authors:
Brenda Carrillo-Conde; Alicia Garza; James Anderegg; Balaji Narasimhan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  95     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-26     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  40-8     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Affiliation:
Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, USA.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
Biocompatible Materials / chemistry*,  metabolism*
Blood Proteins / metabolism*
Cattle
Electrophoresis, Polyacrylamide Gel
Fibrinogen / metabolism
Immunoglobulin G / metabolism
Microscopy, Electron, Scanning
Microspheres*
Ovalbumin / metabolism
Photoelectron Spectroscopy
Polyanhydrides / chemistry*,  metabolism*
Serum Albumin, Bovine / metabolism
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Blood Proteins; 0/Immunoglobulin G; 0/Polyanhydrides; 0/Serum Albumin, Bovine; 9001-32-5/Fibrinogen; 9006-59-1/Ovalbumin

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