Document Detail


Protein adaptation to low temperatures: a comparative study of alpha-tubulin sequences in mesophilic and psychrophilic algae.
MedLine Citation:
PMID:  10484178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The alpha-tubulin genes from two psychrophilic algae belonging to the genus Chloromonas (here named ANT1 and ANT3) have been isolated and sequenced. The genes ant1 and ant3 contain 4 and 2 introns, respectively. The coding DNA sequences are 90% identical but the degree of isology is very high at the polypeptide level (more than 97% strict identities). The ANT1 and ANT3 alpha-tubulin amino acid sequences were compared to the corresponding sequence of the mesophilic alga Chlamydomonas reinhardtii. Of the 15 substitutions detected in ANT1 and/or ANT3, 5 are common to both psychrophilic algae. The recorded substitutions have been analyzed in terms of cold adaptation on the basis of the available three-dimensional structure of the alpha,beta-tubulin heterodimer from pig brain. Most of these are subtle changes, but two substitutions, M268V and A295V occurring in the region of interdimer contacts, could be of great significance for the cold stability of Antarctic algae microtubules due to the fact that the entropic control of microtubule assembly is particularly high in cold adaptes species.
Authors:
S Willem; M Srahna; N Devos; C Gerday; R Loppes; R F Matagne
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  3     ISSN:  1431-0651     ISO Abbreviation:  Extremophiles     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-10-14     Completed Date:  1999-10-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM; S    
Affiliation:
Department of Plant Biology, University of Liège, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Algae, Green / genetics*
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cold Temperature*
DNA
Molecular Sequence Data
Phylogeny
Sequence Homology, Amino Acid
Tubulin / chemistry,  genetics*
Chemical
Reg. No./Substance:
0/Tubulin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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