Document Detail

Protein Ingestion Increases Myofibrillar Protein Synthesis after Concurrent Exercise.
MedLine Citation:
PMID:  24870574     Owner:  NLM     Status:  Publisher    
PURPOSE: We determined the effect of protein supplementation on anabolic signaling and rates of myofibrillar and mitochondrial protein synthesis after a single bout of concurrent training.
METHODS: Using a randomized cross-over design, 8 healthy males were assigned to experimental trials consisting of resistance exercise (8 × 5 leg extension, 80% 1-RM) followed by cycling (30 min at ~70% VO2peak) with either post-exercise protein (PRO: 25 g whey protein) or placebo (PLA) ingestion. Muscle biopsies were obtained at rest, 1 and 4 h post-exercise.
RESULTS: Akt and mTOR phosphorylation increased 1 h after exercise with PRO (175-400%, P<0.01) and was different from PLA (150-300%, P<0.001). MuRF1 and Atrogin-1 mRNA were elevated post-exercise but were higher with PLA compared to PRO at 1 h (50-315%, P<0.05), while PGC-1α mRNA increased 4 h post-exercise (620-730%, P<0.001) with no difference between treatments. Post-exercise rates of myofibrillar protein synthesis increased above rest in both trials (75-145%, P <0.05) but were higher with PRO (67%, P<0.05) while mitochondrial protein synthesis did not change from baseline.
CONCLUSION: Our results show that a concurrent training session promotes anabolic adaptive responses and increases metabolic/oxidative mRNA expression in skeletal muscle. Protein ingestion after combined resistance and endurance exercise enhances myofibrillar protein synthesis and attenuates markers of muscle catabolism and thus is likely an important nutritional strategy to enhance adaptation responses with concurrent training.
Donny M Camera; Daniel W D West; Stuart M Phillips; Tracy Rerecich; Trent Stellingwerff; John A Hawley; Vernon G Coffey
Related Documents :
8739114 - Central and peripheral noradrenalin regulation in eating disorders.
9562214 - Effect of carbohydrate supplementation on plasma glutamine during prolonged exercise an...
23672384 - Fatigue-induced alterations of static and dynamic postural control in athletes with a h...
3571934 - Hepatic blood flow determination. a comparison of 99mtc-diethyl-ida and indocyanine gre...
3678214 - Regulation of growth hormone during exercise by oxygen demand and availability.
24292614 - Effects of vibration on flexibility: a meta-analysis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-27
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  -     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Association of Y Balance Test Reach Asymmetry and Injury in Division I Athletes.
Next Document:  Effects of Exercise in the Cold on Ghrelin, PYY and Food Intake in Overweight Adults.