Document Detail

Protein B: a versatile bacterial Fc-binding protein selective for human IgA.
MedLine Citation:
PMID:  1878208     Owner:  NLM     Status:  MEDLINE    
Protein B, a selective bacterial IgA Fc-binding protein isolated from group B streptococci, has been used to quantify fluid phase and immobilized human IgA. Protein B detects both human IgA1 and IgA2 subclasses and is also reactive with secretory IgA. Protein B can be used immobilized to microtiter plates to capture IgA or following biotinylation as a tracer for fluid phase or immobilized human IgA. The studies presented here suggest protein B will prove to be a valuable reagent for quantitative immunochemical procedures involving human IgA antibodies and facilitate a variety of studies of IgA responses in man.
E L Faulmann; J L Duvall; M D Boyle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioTechniques     Volume:  10     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  748-55     Citation Subset:  IM    
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
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MeSH Terms
Antigens, Bacterial / metabolism*
Bacterial Proteins / metabolism*
Enzyme-Linked Immunosorbent Assay / methods
Immunoblotting / methods
Immunoglobulin A / analysis*,  isolation & purification
Immunoglobulin Fc Fragments / metabolism
Streptococcus agalactiae / immunology*
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Immunoglobulin A; 0/Immunoglobulin Fc Fragments; 58-85-5/Biotin

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