Document Detail

Protein Analysis Through Western Blot of Cells Excised Individually from Human Brain and Muscle Tissue.
MedLine Citation:
PMID:  22402104     Owner:  NLM     Status:  Publisher    
Comparing protein levels from single cells in tissue has not been achieved through Western blot. Laser Capture Microdissection allows for the ability to excise single cells from sectioned tissue and compile an aggregate of cells in lysis buffer. In this study we analyzed proteins from cells excised individually from brain and muscle tissue through Western blot. After we excised individual neurons from the substantia nigra of the brain, the accumulated surface area of the individual cells added to 120,000, 24,000, 360,000, 480,000, 600,000 μm(2). We used an optimized Westerm blot protocol to probe for tyrosine hydroxylase in this cell pool. We also took 360,000 μm(2) of astrocytes (1700 cells) and analyzed the specificity of the method. In muscle we were able to analyze the proteins of the five complexes of the electron transport chain through Western Blot from 200 human cells. With this method, we demonstrate the ability to compare cell specific protein levels in the brain and muscle and describe for the first time how to visualize proteins through Western Blot from cells captured individually.
A O Koob; L Bruns; C Prassler; E Masliah; T Klopstock; A Bender
Related Documents :
19332084 - An integrated approach to improved toxicity prediction for the safety assessment during...
16758114 - Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chel...
22809174 - A homeopathic remedy from arnica, marigold, st. john's wort and comfrey accelerates in ...
20505154 - Effect of noncompetitive proteasome inhibition on bortezomib resistance.
25337574 - Cellular dynamics after injection of mesoderm-derived human embryonic kidney 293 cells ...
9591774 - Increased cell growth and tumorigenicity in human prostate lncap cells by overexpressio...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-5
Journal Detail:
Title:  Analytical biochemistry     Volume:  -     ISSN:  1096-0309     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Department of Neurology, Klinikum Grosshadern, University of Munich, Marchinionistr. 23, 81377 Munich, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Koenigs-Knorr reaction of fusel alcohols with methyl (1-bromo-2,3,4-tri-O-acetyl-?-d-glucopyranosid)...
Next Document:  Phylogenetic study of Baylisascaris schroederi isolated from Qinling subspecies of giant panda in Ch...