Document Detail

Protective role of tretinoin and N-acetyl-L-cysteine from antiproliferative action of cigarette smoke extract on alveolar epithelial cells.
MedLine Citation:
PMID:  17718197     Owner:  NLM     Status:  MEDLINE    
To investigate the mechanisms by which tretinoin and N-acetyl-L-cysteine (NAC) reverse the growth inhibition of alveolar epithelial cells induced by cigarette smoke extract (CSE), MTT assay was used to evaluate cell viability. It was observed that both tretinoin and NAC could restore the viability of CSE-inhibited A549 cells. By incubation with fluorescent indicator H2DCFDA, it was documented that CSE-stimulated accumulation of intracellular reactive oxygen species (ROS) was obviously decreased by tretinoin or NAC. Furthermore, using semi-quantitative and real-time quantitative RT-PCR as well as western blot methods, high expression of insulin-like growth factor binding protein-2 (IGFBP-2) in A549 cells treated with CSE was found at both transcriptional and protein levels, and concomitant with the restoration of cell growth after treatment with tretinoin or NAC, down regulation of IGFBP-2 was observed. From the present study, it is concluded that both RA and NAC can antagonize CSE-induced growth arrest of alveolar epithelial cells and that down regulation of IGFBP-2 may play an important role in the process.
Z Jiao; Z Qu; X Ge; Q Ao; M Xiong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Die Pharmazie     Volume:  62     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-08-27     Completed Date:  2007-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  539-43     Citation Subset:  IM    
Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, RR China.
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MeSH Terms
Acetylcysteine / pharmacology*
Antineoplastic Agents / pharmacology*
Blotting, Western
Cell Proliferation / drug effects*
Cell Survival / drug effects
Down-Regulation / drug effects
Epithelial Cells / drug effects
Free Radical Scavengers / pharmacology*
Indicators and Reagents
Insulin-Like Growth Factor Binding Protein 2 / antagonists & inhibitors,  biosynthesis
Pulmonary Alveoli / cytology*,  drug effects
Reactive Oxygen Species / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Smoke / adverse effects*
Tobacco / chemistry*
Tretinoin / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Free Radical Scavengers; 0/Indicators and Reagents; 0/Insulin-Like Growth Factor Binding Protein 2; 0/Reactive Oxygen Species; 0/Smoke; 302-79-4/Tretinoin; 616-91-1/Acetylcysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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