Document Detail

Protective role and regulation of Rad9 from the fission yeast Schizosaccharomyces pombe.
MedLine Citation:
PMID:  17725619     Owner:  NLM     Status:  MEDLINE    
To assess novel cellular roles and regulation of Rad9 in the fission yeast Schizosaccharomyces pombe, the full-length rad9 gene was cloned into the shuttle vector pRS316, generating pYFRad9. The rad9 mRNA level was significantly increased in the S. pombe cells harboring the plasmid pYFRad9, suggesting that the cloned rad9 gene is functioning. The S. pombe cells harboring pYFRad9 showed higher survival in the minimal media containing nitric oxide (NO)-generating sodium nitroprusside (SNP, 20 muM) and no nitrogen than the vector control cells. SNP and nitrogen starvation notably enhanced the synthesis of beta-galactosidase from the rad9-lacZ fusion gene in the Pap1-positive cells but not in the Pap1-negative cells. The rad9 mRNA level, detected by semi-quantitative reverse transcriptase (RT)-PCR, was elevated in the Pap1-positive cells but not in the Pap1-negative cells by SNP and nitrogen starvation. It was also increased only in the Pap1-positive cells by diethylmaleate, which activates Pap1. Collectively, the results imply that Rad9 plays a protective role against nitrosative and nutritional stress and is positively regulated by NO and nitrogen starvation in a Pap1-dependent manner.
Min-Hee Kang; Eun-Hee Park; Chang-Jin Lim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-24
Journal Detail:
Title:  FEMS microbiology letters     Volume:  275     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-27     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  270-7     Citation Subset:  IM    
Division of Life Sciences and Research Institute of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, Korea.
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MeSH Terms
Basic-Leucine Zipper Transcription Factors / genetics,  metabolism*
Cell Cycle Proteins / genetics,  metabolism*
DNA, Fungal / analysis
Gene Expression Regulation, Fungal*
Heat-Shock Response
Leucine Zippers
Molecular Sequence Data
Nitric Oxide / metabolism,  pharmacology*
Nitrogen / metabolism,  pharmacology*
Schizosaccharomyces / genetics,  growth & development,  metabolism*,  physiology
Schizosaccharomyces pombe Proteins / genetics,  metabolism*
Sequence Analysis, DNA
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/Cell Cycle Proteins; 0/DNA, Fungal; 0/Pap1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 10102-43-9/Nitric Oxide; 139691-42-2/rad9 protein; 7727-37-9/Nitrogen

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