| Protective role and regulation of Rad9 from the fission yeast Schizosaccharomyces pombe. | |
| | |
MedLine Citation:
|
PMID: 17725619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To assess novel cellular roles and regulation of Rad9 in the fission yeast Schizosaccharomyces pombe, the full-length rad9 gene was cloned into the shuttle vector pRS316, generating pYFRad9. The rad9 mRNA level was significantly increased in the S. pombe cells harboring the plasmid pYFRad9, suggesting that the cloned rad9 gene is functioning. The S. pombe cells harboring pYFRad9 showed higher survival in the minimal media containing nitric oxide (NO)-generating sodium nitroprusside (SNP, 20 muM) and no nitrogen than the vector control cells. SNP and nitrogen starvation notably enhanced the synthesis of beta-galactosidase from the rad9-lacZ fusion gene in the Pap1-positive cells but not in the Pap1-negative cells. The rad9 mRNA level, detected by semi-quantitative reverse transcriptase (RT)-PCR, was elevated in the Pap1-positive cells but not in the Pap1-negative cells by SNP and nitrogen starvation. It was also increased only in the Pap1-positive cells by diethylmaleate, which activates Pap1. Collectively, the results imply that Rad9 plays a protective role against nitrosative and nutritional stress and is positively regulated by NO and nitrogen starvation in a Pap1-dependent manner. |
| | |
Authors:
|
Min-Hee Kang; Eun-Hee Park; Chang-Jin Lim |
Related Documents
:
|
6156989 - Interferon inhibits the redistribution of cell surface components. 21330319 - Expression and effects of inhibition of igf-ir tyrosine kinase in mantle cell lymphoma. 15619289 - Rd114-pseudotyped retroviral vectors kill cancer cells by syncytium formation and enhan... 15268779 - Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fet... 3566959 - The immediate induction of extensive cell fusion by ca2+ addition in dictyostelium disc... 16378629 - The cowpox virus fusion regulator proteins spi-3 and hemagglutinin interact in infected... 10825159 - The drosophila caspase dronc cleaves following glutamate or aspartate and is regulated ... 8107199 - Neutralization sensitivity of human immunodeficiency virus type 1 is determined in part... 11861369 - Trk receptor inhibition induces apoptosis of proliferating but not quiescent human oste... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-24 |
Journal Detail:
|
Title: FEMS microbiology letters Volume: 275 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-09-27 Completed Date: 2007-12-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
|
Languages: eng Pagination: 270-7 Citation Subset: IM |
Affiliation:
|
Division of Life Sciences and Research Institute of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, Korea. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/EF646826 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Basic-Leucine Zipper Transcription Factors
/
genetics,
metabolism* Cell Cycle Proteins / genetics, metabolism* DNA, Fungal / analysis Gene Expression Regulation, Fungal* Heat-Shock Response Leucine Zippers Molecular Sequence Data Nitric Oxide / metabolism, pharmacology* Nitrogen / metabolism, pharmacology* Schizosaccharomyces / genetics, growth & development, metabolism*, physiology Schizosaccharomyces pombe Proteins / genetics, metabolism* Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
|
0/Basic-Leucine Zipper Transcription Factors; 0/Cell Cycle Proteins; 0/DNA, Fungal; 0/Pap1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 10102-43-9/Nitric Oxide; 139691-42-2/rad9 protein; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of a new hemolysin from diarrheal isolate SSU of Aeromonas hydrophila.
Next Document: Evaluation of phoP and rpoS mutants of Salmonella enterica serovar Typhi as attenuated typhoid vacci...