| Protective potential of trans-resveratrol against 4-hydroxynonenal induced damage in PC12 cells. | |
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MedLine Citation:
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PMID: 20600804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of 4-hydroxynonenal (4-HNE), a byproduct of membrane lipid peroxidation has been suggested in neurodegeneration. However, the underlying mechanisms are poorly understood. The investigations were carried out to study the preventive potential of trans-resveratrol against 4-HNE induced damage in PC12 cells. Trans-resveratrol, a natural compound obtained from grape skin and found in red wine, is reported to have wide pharmacological window. Cells pretreated with trans-resveratrol (5, 10 and 25 microM) for 24 h were exposed to 4-HNE (25 microM) for 2 h. Pre-treatment of trans-resveratrol was found to be significantly effective in countering the cytotoxic responses of 4-HNE. Significant reduction in reactive oxygen species generation, restoration of intracellular glutathione, and lipid peroxidation levels suggest the improved antioxidant defense system in the cells pretreated with trans-resveratrol. Further, 4-HNE induced alterations in the protein expression of mitochondria-mediated apoptosis markers (Bax, Bcl-2 and Caspase-3) were significantly restored by pre-treatment of trans-resveratrol suggesting the protective potential of trans-resveratrol in PC12 cells against 4-HNE induced oxidative damage. Together these data show the prophylactic potential of trans-resveratrol in oxidative stress mediated apoptotic neurodegeneration. |
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Authors:
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M A Siddiqui; M P Kashyap; V Kumar; A A Al-Khedhairy; J Musarrat; A B Pant |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-22 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-31 Completed Date: 2010-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1592-8 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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DNA Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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toxicity* Animals Antioxidants / pharmacology* Apoptosis / drug effects Apoptosis Regulatory Proteins / metabolism Cell Survival / drug effects Cysteine Proteinase Inhibitors / toxicity* Cytoprotection* Drug Antagonism Glutathione / metabolism Lipid Peroxidation / drug effects Neurons / drug effects*, metabolism, pathology Oxidative Stress / drug effects PC12 Cells Rats Reactive Oxygen Species / metabolism Stilbenes / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Antioxidants; 0/Apoptosis Regulatory Proteins; 0/Cysteine Proteinase Inhibitors; 0/Reactive Oxygen Species; 0/Stilbenes; 29343-52-0/4-hydroxy-2-nonenal; 501-36-0/resveratrol; 70-18-8/Glutathione |
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