Document Detail


Protective immunity against the gastrointestinal nematode Nippostrongylus brasiliensis requires a broad T-cell receptor repertoire.
MedLine Citation:
PMID:  21896015     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The parasitic gastrointestinal nematode Nippostrongylus brasiliensis induces massive expansion of T helper type 2 (Th2) cells in the lung and small intestine. Th2 cells are a major source of interleukin-4 and interleukin-13, two cytokines that appear essential for rapid worm expulsion. It is unclear whether all Th2 cells induced during infection are pathogen-specific because Th2 cells might also be induced by parasite-derived superantigens or cytokine-mediated bystander activation. Bystander Th2 polarization could explain the largely unspecific B-cell response during primary infection. Furthermore, it is not known whether protective immunity depends on a polyclonal repertoire of T-cell receptor (TCR) specificities. To address these unresolved issues, we performed adoptive transfer experiments and analysed the TCR-Vβ repertoire before and after infection of mice with the helminth N. brasiliensis. The results demonstrate that all Th2 cells were generated by antigen-specific rather than superantigen-driven or cytokine-driven activation. Furthermore, we show that worm expulsion was impaired in mice with a limited repertoire of TCR specificities, indicating that a polyclonal T-cell response is required for protective immunity.
Authors:
Alexander Seidl; Marc Panzer; David Voehringer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunology     Volume:  134     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  214-23     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.
Affiliation:
Institute for Immunology, Ludwig-Maximilians-University, Munich Department of Infection Biology, Institute for Microbiology, Universitätsklinikum Erlangen and Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.
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