Document Detail


Protective effects of standardized pomegranate (Punica granatum L.) polyphenolic extract in ultraviolet-irradiated human skin fibroblasts.
MedLine Citation:
PMID:  18717570     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exposure to ultraviolet (UV) radiation has been associated with several acute and chronic conditions, including sunburn, edema, hyperplasia, immunosuppression, photoaging, and skin cancer. The role of naturally occurring phytochemicals in the prevention of such UV-related conditions has captured increased interest. Pomegranate ( Punica granatum L.) is a rich source of polyphenolics, which have been shown to exert anti-inflammatory, antioxidant, and anticarcinogenic activity in numerous in vivo and in vitro studies. This work investigated potential protective effects of a pomegranate fruit extract standardized to punicalagins against UVA- and UVB-induced damage in SKU-1064 human skin fibroblast cells. Pomegranate extract (PE), in a range from 5 to 60 mg/L, was effective at protecting human skin fibroblasts from cell death following UV exposure, likely related to a reduced activation of the pro-inflammatory transcription factor NF-kappaB, a downregulation of proapoptotic caspase-3, and an increased G0/G1 phase, associated with DNA repair. Higher polyphenolic concentrations (500-10000 mg/L) were needed to achieve a significant reduction in UV-induced reactive oxygen species levels and increased intracellular antioxidant capacity (from 1.9 to 8.6 muM Trolox equivalents/mL). Results from this study demonstrate the protective effects of PE against UVA- and UVB-induced cell damage and the potential use of pomegranate polyphenolics in topical applications.
Authors:
Lisbeth A Pacheco-Palencia; Giuliana Noratto; Lal Hingorani; Stephen T Talcott; Susanne U Mertens-Talcott
Publication Detail:
Type:  Journal Article     Date:  2008-08-22
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-17     Completed Date:  2008-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8434-41     Citation Subset:  IM    
Affiliation:
Department of Nutrition and Food Science, Texas A&M University, College Station, Texas 77843, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Death / drug effects
Cell Line
DNA Repair / drug effects
Fibroblasts
Flavonoids / pharmacology*
Fruit / chemistry*
Humans
Phenols / pharmacology*
Plant Extracts / pharmacology*
Punicaceae / chemistry*
Skin / chemistry,  cytology,  radiation effects*
Skin Diseases / prevention & control
Ultraviolet Rays / adverse effects*
Chemical
Reg. No./Substance:
0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/polyphenols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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