Document Detail

Protective effects of probucol treatment on pancreatic beta-cell function of SZ-induced diabetic APA hamsters.
MedLine Citation:
PMID:  14562608     Owner:  NLM     Status:  MEDLINE    
To clarify whether oxidative stress is involved in the pathogenesis of islet lesions of diabetic animals, the effects of probucol (PB), an antioxidant and anti-hyperlipidemia agent, on the islets in streptozotocin (SZ)-induced diabetic APA hamsters in the acute and chronic phases of diabetes were examined. The control (CB group) and diabetic (SZ group) hamsters were treated with PB (1% in the diet) for 4 weeks from several days after SZ injection as the acute diabetic group, or 8 weeks from 6 weeks after SZ injection as the chronic diabetic group. Glucose tolerance test revealed that PB treatment decreased the high serum glucose level after glucose injection in the diabetic APA hamsters in the acute diabetic phase. Immunohistochemistry revealed that PB treatment significantly increased the percentage of the insulin positive area in the diabetic hamsters pancreata in both the acute and chronic phases. In addition, 4-hydroxy-2-nonenal (4HNE; an oxidative stress marker) positive cells were slightly reduced by PB treatment in the acute diabetic phase. Double-immunostaining for insulin and PCNA (proliferating cell nuclear antigen) revealed that elevation of the percentage of insulin and PCNA double-positive cells against insulin-positive cells was seen in the islets of PB-treated diabetic hamsters, but the difference was not significant compared with untreated diabetic hamsters (p = 0.07). In semi-quantitative RT-PCR, the expression of two genes, Reg (Regenerating gene) and INGAP (islet neogenesis associated protein), in the diabetic APA hamsters was significantly increased compared to the control groups in both diabetic phases. PB treatment significantly reduced Reg expression in the chronic diabetic phase. These data suggest that PB treatment in SZ-injected diabetic hamsters partially restored beta-cell function through acting as an antioxidant and induced higher expression of Reg and INGAP genes in the pancreas of hamsters.
Atsushi Takatori; Etsuko Ohta; Toshiaki Inenaga; Keiko Horiuchi; Yoshiyuki Ishii; Shin-ichi Itagaki; Shigeru Kyuwa; Yasuhiro Yoshikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental animals / Japanese Association for Laboratory Animal Science     Volume:  52     ISSN:  1341-1357     ISO Abbreviation:  Exp. Anim.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-10-17     Completed Date:  2003-11-07     Revised Date:  2014-10-24    
Medline Journal Info:
Nlm Unique ID:  9604830     Medline TA:  Exp Anim     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  317-27     Citation Subset:  IM    
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MeSH Terms
Aldehydes / analysis
Anticholesteremic Agents / pharmacology*
Antioxidants / pharmacology*
Biological Markers / analysis
Diabetes Mellitus, Experimental / physiopathology,  prevention & control*
Islets of Langerhans / drug effects*
Oxidative Stress
Probucol / pharmacology*
Proliferating Cell Nuclear Antigen / analysis
Reg. No./Substance:
0/Aldehydes; 0/Anticholesteremic Agents; 0/Antioxidants; 0/Biological Markers; 0/Proliferating Cell Nuclear Antigen; 29343-52-0/4-hydroxy-2-nonenal; 5W494URQ81/Streptozocin; P3CTH044XJ/Probucol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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