Document Detail


Protective effects of hyperoside (quercetin-3-o-galactoside) to PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide.
MedLine Citation:
PMID:  16271843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reactive oxygen species are believed to play a very significant role in the pathogenesis of several diseases including Alzheimer's disease and Parkinson's disease. It is reported that the crucial balance between reactive oxygen species generation and antioxidant defense is regarded as a force in a wide variety of chronic diseases. In this paper, PC12 cells were used to study the antioxidative effect of hyperoside. The results indicated that hyperoside could effectively protect PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide at 160 microg/ml and 100 microg/ml, respectively. The study also showed that hyperoside was no harmful within the tested concentration range and could easily enter into the PC12 cells. With the increasing concentration of hyperoside, cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide was significantly attenuated and the corresponding extracellular lactate dehydrogenase levels decreased concurrently by pretreatment with hyperoside. The results were proved by flow cytometric detection of apoptotic cells. All the above results showed hyperoside could efficiently prevent the PC12 cells from shrinking and turning against apoptosis induced by hydrogen peroxide and tert-butyl hydroperoxide.
Authors:
Zhiyong Liu; Xinyi Tao; Chongwei Zhang; Yanhua Lu; Dongzhi Wei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-23
Journal Detail:
Title:  Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie     Volume:  59     ISSN:  0753-3322     ISO Abbreviation:  Biomed. Pharmacother.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-11-22     Completed Date:  2006-01-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8213295     Medline TA:  Biomed Pharmacother     Country:  France    
Other Details:
Languages:  eng     Pagination:  481-90     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 200237Meilong Road 130, Box 311#, Shanghai , PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / toxicity*
Apoptosis / drug effects
Flow Cytometry
Hydrogen Peroxide / toxicity*
Molecular Structure
Oxidative Stress / drug effects
PC12 Cells
Protective Agents / pharmacology*
Quercetin / analogs & derivatives*,  chemistry,  pharmacology
Rats
Reactive Oxygen Species / metabolism
tert-Butylhydroperoxide / toxicity*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Protective Agents; 0/Reactive Oxygen Species; 117-39-5/Quercetin; 482-36-0/hyperoside; 75-91-2/tert-Butylhydroperoxide; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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