Document Detail

Protective effects of antioxidants against UVA-induced DNA damage in human skin fibroblasts in culture.
MedLine Citation:
PMID:  9860045     Owner:  NLM     Status:  MEDLINE    
Ultraviolet A radiation (UVA, 320-400 nm) is mutagenic and induces genomic damage to skin cells. N-acetyl-cysteine (NAC), selenium and zinc have been shown to have antioxidant properties and to exhibit protective effects against UVA cytotoxicity. The present work attempts to delineate the effect of these compounds on genomic integrity of human skin fibroblasts exposed to UVA radiation using the single cell gel electrophoresis (SCGE) or Comet assay. The cells were incubated with NAC (5 mM), sodium selenite (0.6 microM) or zinc chloride (100 microM). Then cells were embedded in low melting point agarose, and immediately submitted to UVA fluences ranging from 1 to 6J/cm2. In the Comet assay, the tail moment increased by 45% (1 J/cm2) to 89% (6J/cm2) in non-supplemented cells (p)<0.01). DNA damage was significantly prevented by NAC, Se and Zn, with a similar efficiency from 1 to 4J/cm2 (p < 0.05). For the highest UVA dose (6J/cm2), Se and Zn were more effective than NAC (p < 0.01).
N Emonet-Piccardi; M J Richard; J L Ravanat; N Signorini; J Cadet; J C Béani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  29     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-02-23     Completed Date:  1999-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  307-13     Citation Subset:  IM    
LBSO/LCR7 No. 817, Université Joseph Fourier, Grenoble, France.
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MeSH Terms
Acetylcysteine / pharmacology
Antioxidants / pharmacology*
Cells, Cultured
Chlorides / pharmacology
DNA Damage / drug effects*
Electrophoresis, Agar Gel
Fibroblasts / drug effects
Skin / cytology,  drug effects*,  radiation effects
Sodium Selenite / pharmacology
Ultraviolet Rays*
Zinc Compounds / pharmacology
Reg. No./Substance:
0/Antioxidants; 0/Chlorides; 0/Zinc Compounds; 10102-18-8/Sodium Selenite; 616-91-1/Acetylcysteine; 7646-85-7/zinc chloride

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