| Protective effects of antioxidants against UVA-induced DNA damage in human skin fibroblasts in culture. | |
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MedLine Citation:
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PMID: 9860045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ultraviolet A radiation (UVA, 320-400 nm) is mutagenic and induces genomic damage to skin cells. N-acetyl-cysteine (NAC), selenium and zinc have been shown to have antioxidant properties and to exhibit protective effects against UVA cytotoxicity. The present work attempts to delineate the effect of these compounds on genomic integrity of human skin fibroblasts exposed to UVA radiation using the single cell gel electrophoresis (SCGE) or Comet assay. The cells were incubated with NAC (5 mM), sodium selenite (0.6 microM) or zinc chloride (100 microM). Then cells were embedded in low melting point agarose, and immediately submitted to UVA fluences ranging from 1 to 6J/cm2. In the Comet assay, the tail moment increased by 45% (1 J/cm2) to 89% (6J/cm2) in non-supplemented cells (p)<0.01). DNA damage was significantly prevented by NAC, Se and Zn, with a similar efficiency from 1 to 4J/cm2 (p < 0.05). For the highest UVA dose (6J/cm2), Se and Zn were more effective than NAC (p < 0.01). |
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Authors:
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N Emonet-Piccardi; M J Richard; J L Ravanat; N Signorini; J Cadet; J C Béani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 29 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1999-02-23 Completed Date: 1999-02-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 307-13 Citation Subset: IM |
Affiliation:
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LBSO/LCR7 No. 817, Université Joseph Fourier, Grenoble, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Adult Antioxidants / pharmacology* Breast Cells, Cultured Chlorides / pharmacology DNA Damage / drug effects* Electrophoresis, Agar Gel Female Fibroblasts / drug effects Humans Skin / cytology, drug effects*, radiation effects Sodium Selenite / pharmacology Ultraviolet Rays* Zinc Compounds / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Chlorides; 0/Zinc Compounds; 10102-18-8/Sodium Selenite; 616-91-1/Acetylcysteine; 7646-85-7/zinc chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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