| Protective effects of N-acetylcysteine against cadmium-induced damage in cultured rat normal liver cells. | |
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MedLine Citation:
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PMID: 21125209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the protective effects of N-acetylcysteine (NAC), a precursor of reduced glutathione, were studied by measuring the viability, the levels of antioxidant enzymes, and by analyzing the cell cycle in cadmium (Cd)-treated rat liver cells. The cells were treated with 150 µM CdCl2 alone or co-treated with 150 µM CdCl2 and 5 mM NAC (2 h pre-, simultaneous or 2 h post-treatment) for 24 h. The viability of the cells treated with 150 µM CdCl2 alone decreased to 40.1%, while that of the cells co-treated with 5 mM NAC (pre-, simultaneous and post-treatment) significantly increased to 83.7, 86.2 and 83.7%, respectively in comparison to the control cells (100%). The catalase enzyme level decreased to undetectable level in the cells treated with CdCl2 alone, while it significantly increased in the co-treated cells (pre-, simultaneous and post-treatment) to 40.1, 34.3 and 13.2%, respectively. In the cells treated with CdCl2 alone, the glutathione peroxidase enzyme level decreased to 78.3%, while it increased in the co-treated cells (pre-, simultaneous, and post-treatment) to 84.5, 83.3 and 87.9%, respectively. The glutathione reductase enzyme level decreased to 56.1% in the cells treated with cadmium alone, but significantly increased in the cells co treated with NAC (pre-, simultaneous and post-treatment) to 79.5, 78.5 and 78.2%, respectively. Cd caused cell cycle arrest at the S and G2/M phases. The co-treatment with NAC inhibited cell cycle arrest by shifting the cells to the G1 phase. These results clearly show the protective effects of NAC against Cd-induced damage in rat liver cells. |
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Authors:
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Caroline O Odewumi; Veera L D Badisa; Uyen T Le; Lekan M Latinwo; Christopher O Ikediobi; Ramesh B Badisa; Selina F Darling-Reed |
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Publication Detail:
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Type: Journal Article Date: 2010-12-01 |
Journal Detail:
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Title: International journal of molecular medicine Volume: 27 ISSN: 1791-244X ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2010-12-24 Completed Date: 2011-03-31 Revised Date: 2012-04-12 |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 243-8 Citation Subset: IM |
Affiliation:
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Department of Biology, Florida A&M University, Tallahassee, FL 32307, USA. caroline.odewumi@famu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology* Animals Cadmium / toxicity Catalase / metabolism Cell Cycle / drug effects Cell Line Cell Survival / drug effects Gene Expression Regulation, Enzymologic / drug effects Glutathione Peroxidase / metabolism Glutathione Reductase / metabolism Hepatocytes / cytology, drug effects*, metabolism Rats |
| Grant Support | |
ID/Acronym/Agency:
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G12 RR003020-27/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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616-91-1/Acetylcysteine; 7440-43-9/Cadmium; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.8.1.7/Glutathione Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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