| Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion. | |
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MedLine Citation:
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PMID: 22562802 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca(2+)](i)) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10-40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca(2+)](i) elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury. |
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Authors:
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Chen Rui; Li Yuxiang; Hao Yinju; Zhu Qingluan; Wu Yang; Zhao Qipeng; Wang Hao; Ma Lin; Liu Juan; Zhao Chengjun; Jiang Yuanxu; Wang Yanrong; Dai Xiuying; Zhang Wannian; Sun Tao; Yu Jianqiang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-5 |
Journal Detail:
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Title: Journal of molecular histology Volume: - ISSN: 1567-2387 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101193653 Medline TA: J Mol Histol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Pharmacology, Ningxia Medical University, Yinchuan, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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