| Protective effect of metallothioneins against oxidative stress evaluated on wild type and MT-null cell lines by means of flow cytometry. | |
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MedLine Citation:
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PMID: 15259356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is generally accepted that metallothioneins (MTs) are devoted to the regulation of the metabolism of essential trace metals and to chelation of toxic metals. Nowadays, there is increasing evidence that MTs also act as free radical scavengers. We employed wild type mouse embryo fibroblast cell line, GKA1, and its MT-null variant, GKA2, in order to correlate the presence of MTs to the sensitivity of cells to reactive oxygen species (ROS), spontaneously generated by the aerobic cellular metabolism, or chemically induced by hydrogen peroxide. The absence of MTs in GKA2 cells was unambiguously correlated to higher sensitivity to ROS attack, as evaluated by detection and quantification of 8-oxo-2'-deoxyguanosine (8-oxo-G), the first product of oxidative attack to DNA, using Fluorescence-Activated Cell Sorter (FACS). When compared to MT-null cell line, the wild type cells (GKA1) were less sensitive to ROS attack. In GKA1 cells, MT biosynthesis is readily induced by Cd2+ treatment, and such an induction caused a further decrease in sensitivity to ROS injury. On the contrary, the MT-null cells (GKA2) expressed no detectable metallothioneins either constitutively, or after heavy metal pretreatment. Indeed, in GKA2 cell line, pretreatment with Cd2+ did not reduce but even enhanced the oxidative stress. |
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Authors:
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D Colangelo; H Mahboobi; A Viarengo; D Osella |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine Volume: 17 ISSN: 0966-0844 ISO Abbreviation: Biometals Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-07-19 Completed Date: 2005-02-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9208478 Medline TA: Biometals Country: Netherlands |
Other Details:
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Languages: eng Pagination: 365-70 Citation Subset: IM |
Affiliation:
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Dipartimento di Scienze Mediche, Università del Piemonte Orientale 'A. Avogadro', Via Solaroli 17, 28100 Novara, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cadmium / pharmacology Cell Line Cell Survival / drug effects DNA Damage / drug effects Fibroblasts Flow Cytometry Gene Deletion Hydrogen Peroxide / pharmacology Metallothionein / genetics*, metabolism* Mice Oxidative Stress* / drug effects |
| Chemical | |
Reg. No./Substance:
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7440-43-9/Cadmium; 7722-84-1/Hydrogen Peroxide; 9038-94-2/Metallothionein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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