| Protective effect on human lymphocytes of some flavonoids isolated from two Achillea species. | |
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MedLine Citation:
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PMID: 20521537 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 microg/mL) gave a 35.5% decrease in the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 microg/mL and 10 microg/mL) seemed less effective. Flavonoid 5 (3 microg/mL) induced a slight decrease in MN frequency (5%), while higher doses (6 microg/mL and 10 microg/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity. |
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Authors:
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Ivana Aljancić; Miroslava Stanković; Vele Tesević; Ljubodrag Vujisić; Vlatka Vajs; Slobodan Milosavljević |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Natural product communications Volume: 5 ISSN: 1934-578X ISO Abbreviation: Nat Prod Commun Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-06-04 Completed Date: 2010-08-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101477873 Medline TA: Nat Prod Commun Country: United States |
Other Details:
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Languages: eng Pagination: 729-32 Citation Subset: IM |
Affiliation:
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Institute for Chemistry, Technology and Metallurgy, University of Belgrade, NjegoSeva 12, 11000 Belgrade, Serbia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Achillea
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chemistry* Analysis of Variance Cell Proliferation / drug effects Chromosome Breakage / drug effects* Cytokinesis / drug effects Dose-Response Relationship, Drug Flavonoids / isolation & purification*, pharmacology* Humans Lymphocytes / drug effects* Male Micronucleus Tests |
| Chemical | |
Reg. No./Substance:
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0/Flavonoids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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