Document Detail

Protective effect of distillate and redistillate of cow's urine in human polymorphonuclear leukocytes challenged with established genotoxic chemicals.
MedLine Citation:
PMID:  15602821     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: From the ancient period cow's urine has been used as a medicine. In Veda, cow's urine was compared to the nectar. In Susrut, several medicinal properties of cow's urine have been mentioned and are known to cause weight loss, reversal of certain cardiac and kidney problems, indigestion, stomach ache, edema, etc. However, the literature and scripture did not mention the antigenotoxic properties of cow's urine. METHODS: In the present investigation, the antigenotoxic/antioxidant properties of cow's urine distillate and redistillate were studied in vitro. The antioxidant status and volatile fatty acid levels were determined. Actinomycin-D (0.1 micromol/L) and hydrogen peroxide (150 micromol/L) were used for inducing DNA strand break with 0.1% DMSO as negative control. Dose for the antigenotoxic effect of cow's urine was chosen from the dose response study carried out earlier. RESULTS: Both actinomycin-D and H202 caused statistically significant DNA unwinding of 80% & 75% respectively (P < 0.001) as revealed by fluorimetric analysis of DNA unwinding (FADU), and the damage could be protected with the redistilled cow's urine distillate (1, 50 & 100 microL) in simultaneous treatment with genotoxic chemicals. CONCLUSION: The redistillate of cow's urine was found to possess total antioxidant status of around 2.6 mmol, contributed mainly by volatile fatty acids (1500 mg/L) as revealed by the GC-MS studies. These fatty acids and other antioxidants might cause the observed protective effects.
K Krishnamurthi; Dipanwita Dutta; S D Sivanesan; T Chakrabarti
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  17     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-12-16     Completed Date:  2005-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  247-56     Citation Subset:  IM    
Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur-440 020, INDIA.
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MeSH Terms
Ammonia / analysis
Antimutagenic Agents / pharmacology*
Antioxidants / pharmacology*
Cells, Cultured
DNA Damage*
Fatty Acids, Volatile / analysis
Gas Chromatography-Mass Spectrometry
Hydrogen Peroxide
Neutrophils / drug effects*
Urine* / chemistry
Reg. No./Substance:
0/Antimutagenic Agents; 0/Antioxidants; 0/Fatty Acids, Volatile; 0/Mutagens; 50-76-0/Dactinomycin; 7664-41-7/Ammonia; 7722-84-1/Hydrogen Peroxide

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