Document Detail


Protective effect of carbidopa on hydroxyl radical generation in the rat striatum by dopamine.
MedLine Citation:
PMID:  9014169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of L-3,4-dihydroxyphenylalanine (L-DOPA) on the generation of hydroxyl free radicals (.OH) was investigated using striatal microdialysis technique. Salicylic acid in Ringer's solution (0.5 nmol.microliters-1.min-1) was infused directly through a microdialysis probe to detect the generation of .OH as reflected by the formation of dihydroxybenzoic acids (DHBA) in the striatum. When L-DOPA (0.1 mM; 1 microliter.min-1) was infused in the rat brain, the level of 3,4-dihydroxyphenylacetic acid (DOPAC) gradually increased in a time-dependent manner. In addition, a marked elevation of DHBA was observed. However, in the presence of carbidopa, a decarboxylase inhibitor, the elevation in DHBA formation was not observed. These results suggest that carbidopa may suppress the .OH generation by dopamine (DA).
Authors:
T Obata; Y Yamanaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience letters     Volume:  221     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-04-09     Completed Date:  1997-04-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  13-6     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Oita Medical University, Japan.
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MeSH Terms
Descriptor/Qualifier:
3,4-Dihydroxyphenylacetic Acid / metabolism
Animals
Carbidopa / pharmacology*
Chromatography, High Pressure Liquid
Cyclooxygenase Inhibitors / pharmacology
Dopamine / metabolism*
Dopamine Agents / pharmacology*
Free Radicals / metabolism
Hydroxides
Levodopa / pharmacology
Male
Microdialysis
Neostriatum / drug effects,  metabolism*
Neuroprotective Agents / pharmacology
Rats
Rats, Wistar
Sodium Salicylate / pharmacology
Chemical
Reg. No./Substance:
0/Cyclooxygenase Inhibitors; 0/Dopamine Agents; 0/Free Radicals; 0/Hydroxides; 0/Levodopa; 0/Neuroprotective Agents; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 14280-30-9/hydroxide ion; 38821-49-7/Carbidopa; 54-21-7/Sodium Salicylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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