Document Detail


Protective effect of Smilax glabra extract against lead-induced oxidative stress in rats.
MedLine Citation:
PMID:  20580805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ETHNOPHARMACOLOGICAL RELEVANCE: Smilax glabra Roxb. is a traditional Chinese herb, the rhizome of Smilax glabra has been used in folk medicine for the treatment of lead poisoning. AIMS OF THE STUDY: The present study was conducted to investigate the protective role of Smilax glabra extract (SGE) individually or combined with meso-2,3-dimercaptosuccinic acid (DMSA) against the effects of lead acetate on oxidative stress and lead burden in rats. MATERIALS AND METHODS: The biochemical parameters and enzymes in different treated rats were determined by commercial kits. The metal concentrations were measured using atomic absorption spectrophotometer. RESULTS: SGE (300 mg/kg) showed very low toxicity to organs in non-lead exposed rats. Administration of SGE individually had no effect on blood zinc protoporphyrin (ZPP) level but significantly enhanced the glutathione (GSH) content and delta-aminolevulinic acid dehydratase (ALAD), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in lead exposed rats. The co-treatment of SGE and DMSA had a synergism in increasing brain, liver and kidney superoxide dismutase (SOD), catalase (CAT) activities and GSH level, and decreasing oxidized glutathione (GSSG) and thiobarbituric acid reactive substances (TBARS) levels. Moreover, the co-treatment could improve the hepatic and renal histopathology changes. SGE as chelating agent showed significant efficiency in reducing blood and tissue lead burden. CONCLUSIONS: The in vivo results suggested that SGE individually or combined with DMSA exhibited remarkable protective effects on lead-induced oxidative stress and lead burden in rats.
Authors:
Daozong Xia; Xinfen Yu; Sipei Liao; Qijia Shao; Huili Mou; Wei Ma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-24
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  130     ISSN:  1872-7573     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  414-20     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Binjiang District, Hangzhou, China. xdz_zjtcm@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Alanine Transaminase / blood
Alkaline Phosphatase / blood
Animals
Antidotes / pharmacology
Antioxidants / pharmacology*
Body Burden
Brain / drug effects,  metabolism
Catalase / metabolism
Chelating Agents / pharmacology
Disease Models, Animal
Flavonoids / analysis
Glutathione / blood
Kidney / drug effects,  metabolism
Lead Poisoning / drug therapy*,  etiology,  metabolism
Liver / drug effects,  metabolism
Male
Organometallic Compounds / blood
Oxidative Stress / drug effects*
Phenols / analysis
Plant Extracts / chemistry,  pharmacology*
Porphobilinogen Synthase / blood
Protoporphyrins / blood
Rats
Rats, Wistar
Rhizome
Smilax*
Succimer / pharmacology
Superoxide Dismutase / metabolism
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Antidotes; 0/Antioxidants; 0/Chelating Agents; 0/Flavonoids; 0/Organometallic Compounds; 0/Phenols; 0/Plant Extracts; 0/Protoporphyrins; 0/Thiobarbituric Acid Reactive Substances; 15442-64-5/zinc protoporphyrin; 301-04-2/lead acetate; 304-55-2/Succimer; 70-18-8/Glutathione; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase; EC 2.6.1.2/Alanine Transaminase; EC 3.1.3.1/Alkaline Phosphatase; EC 4.2.1.24/Porphobilinogen Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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