Document Detail


Protective effect of S-allyl-L-cysteine, a garlic compound, on amyloid beta-protein-induced cell death in nerve growth factor-differentiated PC12 cells.
MedLine Citation:
PMID:  12725918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aged garlic extract (AGE) contains several neuroactive compounds, including S-allyl-L-cysteine (SAC) and allixin. We characterized cell death induced by amyloid beta-protein (Abeta), 4-hydroxynonenal (HNE), tunicamycin, an endoplasmic reticulum (ER) stressor, or trophic factor deprivation, and investigated whether and how SAC could prevent this in nerve growth factor (NGF)-differentiated PC12 cells, a model of neuronal cells. Exposure of the cells to amyloid beta-protein(1-40) (Abeta(1-40)) decreased the extent of [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) reduction activity and loss of neuronal integrity, but these effects were not prevented by Ac-DEVD-CHO, a caspase-3 inhibitor. Simultaneously applied SAC protected the cells against Abeta-induced cell death in a concentration-dependent manner. It also protected them against tunicamycin-induced neuronal death. In contrast, it afforded no protection against cell death induced by HNE and trophic factor deprivation, which is mediated by a caspase-3-dependent pathway. These results suggest that SAC may selectively protect cell death induced by Abeta and tunicamycin, which may be triggered by ER dysfunction in NGF-differentiated PC12 cells.
Authors:
Yoshihisa Ito; Yasuhiro Kosuge; Taeko Sakikubo; Kayo Horie; Natsue Ishikawa; Naoya Obokata; Eiko Yokoyama; Kumiko Yamashina; Machiko Yamamoto; Hiroshi Saito; Motoki Arakawa; Kumiko Ishige
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  46     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-02     Completed Date:  2003-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  119-25     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba 274-8555, Japan. yoshiito@pha.nihon-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Aldehydes / toxicity
Amyloid beta-Protein / toxicity*
Animals
Anti-Bacterial Agents / toxicity
Cell Death / drug effects*
Cell Differentiation / drug effects
Cysteine / analogs & derivatives*,  pharmacology*
Cysteine Proteinase Inhibitors / toxicity
Endoplasmic Reticulum / physiology
Garlic
Nerve Growth Factor / pharmacology
Neurons / drug effects*
Neuroprotective Agents / pharmacology*
Oligopeptides / toxicity
PC12 Cells
Rats
Tunicamycin / toxicity
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Amyloid beta-Protein; 0/Anti-Bacterial Agents; 0/Cysteine Proteinase Inhibitors; 0/Neuroprotective Agents; 0/Oligopeptides; 0/acetyl-aspartyl-glutamyl-valyl-aspartal; 11089-65-9/Tunicamycin; 21593-77-1/S-allylcysteine; 29343-52-0/4-hydroxy-2-nonenal; 52-90-4/Cysteine; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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