Document Detail

Protective effect of Portulaca oleracea extracts on hypoxic nerve tissue and its mechanism.
MedLine Citation:
PMID:  17392109     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to investigate whether Portulaca oleracea (PO) extracts have hypoxic neuroprotective effects and if so, by what mechanism. After being orally administrated with the PO extracts or distilled water for seven days, adult male BALB/c mice were adapted to a normobaric low oxygen environment (10% oxygen and 90% nitrogen) for different time and then were sacrificed. The mouse cortices were used for histological analysis by hematoxylin and eosin (H-E staining). The activities of pyruvate kinase (PK), phosphofructokinase (PFK), lactic acid (LD) and the level of lactate dehydroenase (LDH) and ATP were detected, and the mRNA and protein levels of EPO in the cortices were analyzed. PC-12 cells and primarily cultured nerve cells were used for 3-(4,5-Dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. The degree of LDH in the cell culture medium was tested. The results showed that the PO extracts enhanced the EPO mRNA and protein expression in the mouse cortices. Compared to the control group, the mouse in the group treated with the PO extracts by 1 g/d had significantly higher activities of PF, PFK, LDH and higher levels of ATP in the cortices, especially under the hypoxic environment for 24 hours. Histological analysis indicated that the extracts lessened the inflammation damage of the mouse brain. MTT assay results showed the PO extracts or the herb-containing serum raised the viability of the cells under the tested hypoxic conditions and decreased the degree of LDH in the culture medium in a dose-dependent manner. We thus demonstrated that the PO extracts had protective effects on hypoxic nerve tissue.
Wanyin Wang; Limin Gu; Liwei Dong; Xiaoli Wang; Changquan Ling; Min Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Asia Pacific journal of clinical nutrition     Volume:  16 Suppl 1     ISSN:  0964-7058     ISO Abbreviation:  Asia Pac J Clin Nutr     Publication Date:  2007  
Date Detail:
Created Date:  2007-03-29     Completed Date:  2007-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9440304     Medline TA:  Asia Pac J Clin Nutr     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Department of Naval Medicine, Second Military Medical University, Shanghai, China 200433.
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MeSH Terms
Cerebral Cortex / drug effects*,  enzymology*
Dose-Response Relationship, Drug
L-Lactate Dehydrogenase / metabolism
Lactic Acid / metabolism
Mice, Inbred BALB C
Neuroprotective Agents / pharmacology*
Phosphofructokinases / metabolism
Plant Extracts / pharmacology*
Portulaca / chemistry*
Pyruvate Kinase / metabolism
Random Allocation
Reg. No./Substance:
0/Neuroprotective Agents; 0/Plant Extracts; 50-21-5/Lactic Acid; EC Dehydrogenase; EC 2.7.1 -/Phosphofructokinases; EC Kinase

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