| Protective effect of PEG 35,000 Da on renal cells: paradoxical activation of JNK signaling pathway during cold storage. | |
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MedLine Citation:
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PMID: 16827853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polyethylene glycol (PEG), a high-molecular weight colloid, is added to preservation solutions in order to decrease cold- and ischemia-induced injuries of the grafted organ. We evaluated on LLC-PK1, a porcine proximal tubular epithelial cell line (1) the efficiency of several commercial preservation solutions (University of Wisconsin, Euro-Collins, Celsior, SCOT, IGL-1), and (2) whether adding PEG (400-35,000 Da) in a simple extracellular-type buffer modified cell integrity and mitogen-activated protein kinase (MAPK) signaling pathways. SCOT was the most efficient commercial solution. Moreover, only PEG 35,000 Da totally preserved cell viability, induced a decrease on reactive oxygen species production and a decrease on p38-MAPK activation. Furthermore PEG 35,000 Da stimulated c-Jun N-terminal kinase (JNK). However, the inhibition of JNK pathway, with the specific SP600125 inhibitor, in the presence of PEG 35,000 Da did not affect cell survival. We also confirmed on whole pig kidney the protective effect of PEG 35,000 Da on cold-induced tubular injuries. This study confirms PEG antioxidative properties, but we demonstrate that its effect on JNK signaling pathway had also a paradoxical effect on cell death. This sheds a new light on PEG effects during cell preservation, independently from the classical immuno-camouflaging hypothesis. |
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Authors:
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D Dutheil; I Rioja-Pastor; C Tallineau; J-M Goujon; T Hauet; G Mauco; I Petit-Paris |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons Volume: 6 ISSN: 1600-6135 ISO Abbreviation: Am. J. Transplant. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-10 Completed Date: 2006-12-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100968638 Medline TA: Am J Transplant Country: Denmark |
Other Details:
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Languages: eng Pagination: 1529-40 Citation Subset: IM |
Affiliation:
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Inserm E0324, Poitiers, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Cell Death / drug effects Cells, Cultured Cold Temperature Enzyme Activation / drug effects JNK Mitogen-Activated Protein Kinases / metabolism* Kidney / cytology, drug effects*, enzymology* Lipid Peroxidation / drug effects Microscopy, Electron, Scanning Molecular Weight Organ Preservation Solutions Polyethylene Glycols / pharmacology* Preservation, Biological / methods* Signal Transduction / drug effects* Swine |
| Chemical | |
Reg. No./Substance:
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0/Organ Preservation Solutions; 0/Polyethylene Glycols; 56-65-5/Adenosine Triphosphate; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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