Document Detail

Protective effect of L-glutamine against freeze-thaw damage in mammalian cells.
MedLine Citation:
PMID:  3371057     Owner:  NLM     Status:  MEDLINE    
L-Glutamine at 18 mM protects mammalian cells against freeze-thaw (FT) damage by a factor of about 6, depending on FT conditions, in balanced salt solutions. While not nearly as effective a cryoprotectant as dimethyl sulfoxide (DMSO) or propylene glycol (PG), the mechanism of protection by glutamine appears to be independent from that of DMSO or PG; thus, 18 mM glutamine is effective at reducing FT damage in combination with these agents. These combinations allow lower concentrations of the more toxic agents DMSO and PG to be used in FT medium. There is no pre-FT or post-FT effect of glutamine when cells are exposed to a FT cycle in balanced salt solutions. Hence, protection is due to its presence during the FT-cycle. The presence of 2 mM L-glutamine in Eagle's basal medium is sufficient to account for cryoprotection by this medium.
J Kruuv; D J Glofcheski; J R Lepock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  25     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-06-24     Completed Date:  1988-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  121-30     Citation Subset:  IM    
Guelph-Waterloo Program for Graduate Work in Physics, University of Waterloo, Ontario, Canada.
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MeSH Terms
Cell Line
Cell Survival / drug effects*
Cryoprotective Agents* / pharmacology
Culture Media
Glutamine / pharmacology*
Reg. No./Substance:
0/Cryoprotective Agents; 0/Culture Media; 56-85-9/Glutamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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