Document Detail


Protective effect of the Baicalin against DNA damage induced by ultraviolet B irradiation to mouse epidermis.
MedLine Citation:
PMID:  18717958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate whether topical application of Baicalin affords protecting Balb/C mice epidermis from ultraviolet (UV)B-induced DNA damage and its underlying mechanisms. METHODS: A DNA damage model of UVB irradiation-induced mice epidermis was established. The immunohistochemical staining, Southwestern dot-blotting were used for cyclobutane pyrimidine dimers (CPDs) detection; Western blotting was used for p53 detection; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of Bcl-2 and Bax; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells. RESULTS: Topical application of Baicalin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 1, 24 and 48 h after 180 mJ/cm(2) of UVB irradiation as compared with untreated mice. UVB-induced apoptosis was less pronounced in Baicalin-treated mice epidermis, which was accompanied by less p53 accumulation and higher Bcl-2/Bax ratio compared with that of untreated mice. CONCLUSION: Taken together, these results suggest that topical Baicalin application mitigates DNA photo-damage. Baicalin is therefore a promising protective substance against UVB radiation.
Authors:
Zhou Bing-Rong; Jin Song-Liang; Chen Xiao-E; Lin Xiang-Fei; Cai Bao-Xiang; Gao Jie; Luo Dan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photodermatology, photoimmunology & photomedicine     Volume:  24     ISSN:  1600-0781     ISO Abbreviation:  Photodermatol Photoimmunol Photomed     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-22     Completed Date:  2008-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9013641     Medline TA:  Photodermatol Photoimmunol Photomed     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  175-82     Citation Subset:  IM    
Affiliation:
Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
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MeSH Terms
Descriptor/Qualifier:
Administration, Cutaneous
Analysis of Variance
Animals
Apoptosis / drug effects,  radiation effects
DNA / analysis
DNA Damage / drug effects*
Epidermis / drug effects*,  metabolism,  physiology,  radiation effects*
Female
Flavonoids / pharmacology*
Mice
Mice, Inbred BALB C
Plant Extracts / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Pyrimidine Dimers / metabolism
RNA, Messenger / analysis
Scutellaria baicalensis*
Tumor Suppressor Protein p53 / metabolism
Ultraviolet Rays / adverse effects*
bcl-2-Associated X Protein / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Bax protein, mouse; 0/Flavonoids; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; 0/Pyrimidine Dimers; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 21967-41-9/baicalin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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